Abstract
The effect of cytidine 5′-monophosphate (CMP) on the incorporation of cytidine 5′-diphosphate (CDP) [methyl-14C]choline or [1-14C]dipalmitoylglycerol into phosphatidylcholine (PC) catalyzed by rabbit lung microsomal CDP choline:1,2-diacyl-sn-glycerol cholinephosphotransferase (EC 2.7.8.2) was studied. In the presence of 0.85 mM CMP and nonsaturating diacylglycerol concentration, the incorporation of CDP[14C]choline into PC was markedly stimulated, but the incorporation of [14C]dipalmitoylglycerol into PC was inhibited. This was due to the increase of endogenous diacylglycerol generated from microsomal PC by the cholinephosphotransferase reverse reaction. However, the newly synthesized PC was not readily hydrolyzed in the presence of CMP. The results of this study suggest that the endogenous membranous diacylglycerol is utilized more preferentially for PC synthesis than the exogenous diacylglycerol and that the newly synthesized PC could rapidly equilibrate with the endogenous membrane PC pool.
Similar content being viewed by others
References
Kennedy, E.P. (1960) inEnzymes (Boyer, P.D., Lardy, H., and Myrback, K., eds.) Vol. 2, pp. 63–74, Academic Press, New York.
Weiss, S.G., Smith, S.W., and Kennedy, E.P. (1958)J. Biol. Chem. 231, 53–64.
Kanoh, H., and Ohno, K. (1973)Biochim. Biophys. Acta 306, 203–217.
Kanoh, H., and Ohno, K. (1973)Biochim. Biophys. Acta 326, 17–25.
Goracci, G., Gresele, P., Arienti, G., Porrovecchio, P., Nenci, G., and Porcellati, G. (1983)Lipids 18, 179–185.
Post, M., Batenburg, J.J., Schuurmans, E.A.J.M., and Van Golde, L.M.G. (1982)Biochim. Biophys. Acta 712, 390–394.
Weinhold, P.A., Quade, M.M., Brozowski, T.B., and Feldman, D.A. (1980)Biochim. Biophys. Acta 617, 76–84.
Tsao, F.H.C., and Zachman, R.D. (1977)Pediatr. Res. 11, 849–857.
Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.
Miller, J.C., and Weinhold, P.A. (1981)J. Biol. Chem. 256, 12662–12665.
Kates, M. (1975) inLaboratory Techniques in Biochemistry and Molecular Biology, pp. 479–481, North-Holland Publishing Co., Amsterdam.
Filler, D.A., and Rhoades, R.A. (1982)Exp. Lung Res. 3, 37–46.
Garen, A., and Levinthal, C. (1960)Biochim. Biophys. Acta 38, 470–483.
Masters, B.S.S., Williams, C.H. Jr., and Kamin, H. (1967)Methods Enzymol. 10, 565–573.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.
Sarzala, M.G., and Van Golde, L.M.G. (1976)Biochim. Biophys. Acta 441, 423–432.
Van Heusden, G.P.H., and Van Den Bosch, H. (1982)Biochim. Biophys. Acta 711, 361–368.
Coleman, R., and Bell, R.M. (1977)J. Biol. Chem. 252, 3050–3056.
Goracci, G., Francescangeli, E.L., Horrocks, L.A., and Porcellati, G. (1981)Biochim. Biophys. Acta 664, 373–379.
Ruston, B., and Kunze, D. (1984)Biochim. Biophys. Acta 793, 372–378.
Infante, J.P. (1977)Biochem. J. 167, 847–849.
Sleight, R., and Kent, C. (1980)J. Biol. Chem. 255, 10644–10650.
Vance, D.E., and Choy, P.C. (1979)Trends Biochem. Sci. 4, 145–148.
Tsao, F.H.C.Biol. Neonate, in press.
Author information
Authors and Affiliations
About this article
Cite this article
Tsao, F.H.C. Reversibility of cholinephosphotransferase in lung microsomes. Lipids 21, 498–502 (1986). https://doi.org/10.1007/BF02535636
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02535636