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BW755C or staurosporine inhibits collagen-stimulated phosphoinositide phosphorylation in platelets

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Lipids

Abstract

Stimulation of platelets by collagen results in increased formation of the polyphosphoinositides, phosphatidylinositol phosphate (PtdInsP) and phosphatidylinositol bisphosphate (PtdInsP 2) through stimulation of phosphoinositide kinase activities. We investigated a possible regulatory role of endogenous thromboxane formation and protein kinase C (PKC) activation in the induction of phosphoinositide phosphorylation following collagen stimulation, as well as following stimulation by the thromboxane mimetic, U-46619. Human platelets were prelabeled with [3H]inositol and stimulated with collagen (2 μg/mL) or U-46619 (1 μM), in the absence or presence of either the cyclo-oxygenase/lipoxygenase inhibitor, BW755C, or staurosporine, a putative inhibitor or PKC. Collagen stimulation resulted in a time-dependent increase in [3Hinositol-labeled PtdInsP and PtdInsP 2 which was completely inhibited in the presence of BW755C. Addition of U-46619 to BW755C-treated, collagen-stimulated platelets restored the increased polyphosphoinositide formation. Stimulation of platelets with U-46619 alone also resulted in increased formation of [3H]PtdInsP and [3H]PtdInsP 2, but this was not affected by the presence of BW755C. These results suggest that the collagen-induced activation of phosphoinositide kinases was dependent upon thromboxane formation, but that U-46619-induced phosphoinositide formation was rather independent of further thromboxane production. Pretreatment of platelets with staurosporine, prior to agonist addition, completely blocked the collagen-stimulated rise in radiolabeled PtdInsP and the U-46619-induced PtdInsP and PtdInsP 2 generations, suggesting that protein kinase, possibly PKC, may play a role in the activation of phosphoinositide kinases by these agonists.

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Abbreviations

ANS:

8-anilino-1-naphthalenesulfonic acid

BW755C:

3-amino-1-(3-trifluromethylphenyl)-2-pyrazoline

lysoPtdIns:

lysophosphatidylinositol

PKC:

protein kinase C

PtdIns:

phosphatidylinositol

PtdInsP :

phosphatidylinositol phosphate

PdtInsP 2 :

phosphatidylinositol bisphosphate

U-46619:

9,11-dideoxy-11α,9α-epoxymethanoprostaglandin F2α

References

  1. Verhoeven, A.J.M., Tysnes, O., Aarbakke, G.M., Cook, C.A., and Holmsen, H. (1987)Eur. J. Biochem. 166, 3–9.

    Article  PubMed  CAS  Google Scholar 

  2. Billah, M.M., and Lapetina, E.G. (1982)J. Biol. Chem. 257, 12705–12708.

    PubMed  CAS  Google Scholar 

  3. Imai, A., Nakashima, S., and Nozawa, Y. (1983)Biochem. Biophys. Res. Commun. 110, 108–115.

    Article  PubMed  CAS  Google Scholar 

  4. Mauco, G., Dangelmaier, C.A., and Smith, J.B. (1984)Biochem. J. 224, 933–940.

    PubMed  CAS  Google Scholar 

  5. Tysnes, O., Verhoeven, A.J.M., and Holmsen, H. (1987)Biochem. Biophys. Res. Commun. 144, 454–462.

    Article  PubMed  CAS  Google Scholar 

  6. Narita, H., Park, H.J., Tanaka, K., Matsuura, T., and Kito, M. (1985)J. Biochem. 98, 1063–1068.

    PubMed  CAS  Google Scholar 

  7. Karniguian, A., Rendu, F., Grelac, F., Lebret, M., and Legrand, Y.J. (1987)Biochem. Biophys. Res. Commun. 146, 277–283.

    Article  PubMed  CAS  Google Scholar 

  8. Thomas, L.M., and Holub, B.J. (1987)Lipids 22, 144–147.

    Article  PubMed  CAS  Google Scholar 

  9. Suga, K., Kambayashi, T., Tsujinaka, T., Sakon, M., and Mori, T. (1987)Biochem. Int. 15, 769–777.

    PubMed  CAS  Google Scholar 

  10. Ling, L.E., Schulz, J.T., and Cantley, L.C. (1989)J. Biol. Chem. 264, 5080–5088.

    PubMed  CAS  Google Scholar 

  11. De Chaffroy de Courcelles, D., Roevens, P., and Van Belle, H. (1986)FEBS Lett. 195, 115–118.

    Article  Google Scholar 

  12. Suga, K., Kambayashi, J., Kawasaki, T., Sakon, M., and Mori, T. (1986)Thromb. Res. 44, 155–163.

    Article  PubMed  CAS  Google Scholar 

  13. Halenda, S.P., and Feinstein, M.B. (1984)Biochem. Biophys. Res. Commun. 124, 507–513.

    Article  PubMed  CAS  Google Scholar 

  14. De Chaffroy de Courcelles, D., Roevens, P., and Van Belle, H. (1984)FEBS Lett. 173, 389–393.

    Article  Google Scholar 

  15. De Chaffroy de Courcelles, D., Roevens, P., and Van Belle, H. (1984)Biochem. Biophys. Res. Commun. 123, 589–595.

    Article  Google Scholar 

  16. Strosznajder, J., and Strosznajder, R.P. (1989)Neurochem. Res. 14, 717–723.

    Article  PubMed  CAS  Google Scholar 

  17. Smith, C.D., and Chang, K. (1989)J. Biol. Chem. 264, 3206–3210.

    PubMed  CAS  Google Scholar 

  18. Urumow, T., and Wieland, O.H. (1990)FEBS Lett. 263, 15–17.

    Article  PubMed  CAS  Google Scholar 

  19. Lloyd, J.V., and Mustard, J.F. (1974)Br. J. Haematol. 26, 243–253.

    PubMed  CAS  Google Scholar 

  20. Magósci, M., Enyedi, A., Sarkadi, B., and Gárdos, G. (1988)Biochim. Biophys. Acta 944, 202–212.

    Article  Google Scholar 

  21. Nahas, N., Plantavid, M., Mauco, G., and Chap, H. (1989)FEBS Lett. 246, 30–34.

    Article  PubMed  CAS  Google Scholar 

  22. Chauhan, V.P.S., and Brockerhoff, H. (1988)Biochem. Biophys. Res. Commun. 155, 18–23.

    Article  PubMed  CAS  Google Scholar 

  23. Eberhard, D.A., Cooper, C.L., Low, M.G., and Holz, R.W. (1990)Biochem. J. 268, 15–25.

    PubMed  CAS  Google Scholar 

  24. Watson, S.P., Reep, B., McConnell, R.T., and Lapetina, E.G. (1985)Biochem. J. 226, 831–837.

    PubMed  CAS  Google Scholar 

  25. Siess, W., Cuatrecasas, P., and Lapetina, E.G. (1983)J. Biol. Chem. 258, 4683–4686.

    PubMed  CAS  Google Scholar 

  26. Pollock, W.K., Rink, T.J., and Irvine, R.F. (1986)Biochem. J. 235, 869–877.

    PubMed  CAS  Google Scholar 

  27. Ardlie, N., Garret, J., and Bell, L. (1986)Thromb. Res. 42, 115–124.

    Article  PubMed  CAS  Google Scholar 

  28. Molnar, J., and Lorand, L. (1961)Arch. Biochem. Biophys. 93, 353–363.

    Article  PubMed  CAS  Google Scholar 

  29. Aster, R.J., and Jandl, J.H. (1964)J. Clin. Invest. 43, 843–855.

    PubMed  CAS  Google Scholar 

  30. Mustard, J.F., Perry, D.W., Ardlie, N.G., and Packham, M.A. (1972)Br. J. Haematol. 22, 193–204.

    PubMed  CAS  Google Scholar 

  31. Culty, M., Davidson, M.L., and Haslam, R.J. (1988)Eur. J. Biochem. 171, 523–533.

    Article  PubMed  CAS  Google Scholar 

  32. Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  33. Allan, D., and Michell, R.H. (1978)Biochim. Biophys. Acta 508, 277–286.

    Article  PubMed  CAS  Google Scholar 

  34. Lynch, R.D. (1980)Lipids 15, 412–420.

    Article  PubMed  CAS  Google Scholar 

  35. Steel, R.G.D., and Torrie, J.H. (1980)Principles and Procedures of Statistics, 2nd edn., pp. 86–121, McGraw-Hill, New York.

    Google Scholar 

  36. Higgs, G.A., Flower, R.J., and Vane, J.R. (1979)Biochem. Pharmacol. 28, 1959–1961.

    Article  PubMed  CAS  Google Scholar 

  37. Smith, J.B., Dangelmaier, C., and Mauco, G. (1985)Biochim. Biophys. Acta 835, 344–351.

    PubMed  CAS  Google Scholar 

  38. Vedelago, H.R., and Mahadevappa, V.G. (1988)Biochem. Biophys. Res. Commun. 150, 177–184.

    Article  PubMed  CAS  Google Scholar 

  39. Tamaoki, T., Nomoto, H., Takahashi, I., Kato, Y., Morimoto, M., and Tomita, F. (1986)Biochem. Biophys. Res. Commun. 135, 397–402.

    Article  PubMed  CAS  Google Scholar 

  40. Watson, S., McNally, J., Shipman, L., and Godfrey, P. (1988)Biochem. J. 279, 249–350.

    Google Scholar 

  41. Tysnes, O.-B., Verhoeven, A.J.M., and Holmsen, H. (1988)Eur. J. Biochem. 174, 75–79.

    Article  PubMed  CAS  Google Scholar 

  42. Meyer, T., Regenass, U., Fabbro, D., Alteri E., Rösell, J., Müller, M., Caravatti, G., and Matter, A. (1989)Int. J. Cancer 43, 851–856.

    PubMed  CAS  Google Scholar 

  43. Rüegg, U.T., and Burgess, G.M. (1989)Trends Pharmacol. Sci. 10, 218–220.

    Article  PubMed  Google Scholar 

  44. Bishop, W.R., August, J., Petrin, J.M., and Pai, J.K. (1990)Biochem. J. 269, 465–473.

    PubMed  CAS  Google Scholar 

  45. Crouch, M.F., and Lapetina, E.G. (1989)J. Biol. Chem. 264, 584–588.

    PubMed  CAS  Google Scholar 

  46. King, W.G., and Rittenhouse, S.E. (1989)J. Biol. Chem. 264, 6070–6074.

    PubMed  CAS  Google Scholar 

  47. Hanasaki, K., Nakano, T., and Arita, H. (1987)J. Biochem. 102, 5–8.

    PubMed  CAS  Google Scholar 

  48. Rittenhouse, S.E., and Allen, C.L. (1982)J. Clin. Invest. 70, 1216–1224.

    Article  PubMed  CAS  Google Scholar 

  49. Thomas, L.M., and Holub, B.J. (1991)Biochim. Biophys. Acta 1081, 92–98.

    PubMed  CAS  Google Scholar 

  50. Ahn, C., Romstedt, K.J., Wallace, L.J., Miller, D.D., and Feller, D.R. (1988)Biochem. Pharmacol. 37, 3023–3033.

    Article  PubMed  CAS  Google Scholar 

  51. Vedelago, H.R., and Mahadevappa, V.G. (1988)Biochem. J. 256, 981–987.

    PubMed  CAS  Google Scholar 

  52. Thomas, L.M., and Holub, B.J. (1991)Cellular Signalling 3, 159–167.

    Article  PubMed  CAS  Google Scholar 

  53. Yoshida, K., and Nachmias, V.T. (1987)J. Biol. Chem. 262, 16048–16054.

    PubMed  CAS  Google Scholar 

  54. Kucera, G.L., and Rittenhouse, S.E. (1990)J. Biol. Chem. 265, 5345–5348.

    PubMed  CAS  Google Scholar 

  55. Yamamoto, K., and Lapetina, E.G. (1990)Biochem. Biophys. Res. Commun. 168, 466–472.

    Article  PubMed  CAS  Google Scholar 

  56. Sultan, C., Breton, M., Mauco, G., Grondin, P., Plantavid, M., and Chap, H. (1990)Biochem. J. 269, 831–834.

    PubMed  CAS  Google Scholar 

  57. Nolan, R.D., and Lapetina, E.G. (1990)J. Biol. Chem. 265, 2441–2445.

    PubMed  CAS  Google Scholar 

  58. Van Rooijen, L.A.A., Rossowska, M., and Bazan, N.G. (1985)Biochem. Biophys. Res. Commun. 126, 150–155.

    Article  PubMed  Google Scholar 

  59. Lundberg, G.A., Jergil, B., and Sundler, R. (1986)Eur. J. Biochem. 161, 257–262.

    Article  PubMed  CAS  Google Scholar 

  60. Lassing, I., and Lindberg, U. (1990)FEBS Lett. 262, 231–233.

    Article  PubMed  CAS  Google Scholar 

  61. Lassing, I., and Lindberg, U. (1985)Nature 314, 472–474.

    Article  PubMed  CAS  Google Scholar 

  62. Janmey, P.A., Iida, K., Yin, H.L., and Stossel, T.P. (1987)J. Biol. Chem. 262, 12228–12236.

    PubMed  CAS  Google Scholar 

  63. Alonso, M.T., Sanchez, A., and Garcia-Sancho, J. (1989)Biochem. Biophys. Res. Commun. 162, 24–29.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Lisa M. Thomas & Bruce J. Holub

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  1. Lisa M. Thomas
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Thomas, L.M., Holub, B.J. BW755C or staurosporine inhibits collagen-stimulated phosphoinositide phosphorylation in platelets. Lipids 26, 689–695 (1991). https://doi.org/10.1007/BF02535615

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  • DOI: https://doi.org/10.1007/BF02535615

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