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Evidence for loss of tritium from 3β-tritiated deoxycholic acid during enterohepatic cycling in man

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Lipids

Abstract

A double-label single-intubation isotope technique was assessed for studying deoxycholic acid metabolism by simultaneous IV administration of [3β-3H]deoxycholic acid and [24-14C]deoxycholic acid to five healthy subjects. The3H/14C ratio in duodenal bile fell progressively in all subjects, the mean reduction on day four being 48.8%. When the same labeled bile acids were administered to three ileostomy subjects, again the ratio fell with a mean reduction of 44.4% on day four. This fall more likely was due to hepatic exchange of tritium for hydrogen, rather than to colonic bacterial oxidation. [3β-3H]Deoxycholic acid should not be used to study the kinetics and metabolism of deoxycholic acid.

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Abbreviations

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine, T-O13, University of California, San Diego, 92093, La Jolla, CA

    S. N. Marcus

  2. University Department of Medicine, Bristol Royal Infirmary, BS2 8HW, Bristol, England

    K. W. Heaton

Authors
  1. S. N. Marcus
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  2. K. W. Heaton
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Marcus, S.N., Heaton, K.W. Evidence for loss of tritium from 3β-tritiated deoxycholic acid during enterohepatic cycling in man. Lipids 23, 629–630 (1988). https://doi.org/10.1007/BF02535611

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  • DOI: https://doi.org/10.1007/BF02535611

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