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Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase

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Lipids

Abstract

A fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic-2,3(1,2)-dioleoyl-sn-glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic triacylglycerol lipase activities in post-heparin plasma. Optimal conditions for the determination of the two post-heparin plasma lipases were similar to those using radiolabeled triolein. Using this substrate, both post-heparin lipases exhibited their characteristic properties (pH optimum and effect of inhibitors) and attacked external ester bonds (1 or 3) containing pyrene decanoic and oleic acids at a similar rate

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Abbreviations

LPL:

lipoprotein lipase

H-TGL:

hepatic triacylglycerol lipase

P10OOG:

1(3)pyrene decanoyl-2,3(1,2)-dioleoyl-sn-glycerol

VLDL:

very low density lipoproteins

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Authors and Affiliations

  1. INSERM Unite 101, Biochimie des Lipides, Hôpital Purpan, 31059, Toulouse, France

    Nicole Dousset, Anne Negre, Robert Salvayre, Pierre Rogalle, Quoc Quan Dang & Louis Douste-Blazy

  2. Laboratoire de Biochimie Medicale, Faculte de Medecine Purpan, 31059, Toulouse, France

    Nicole Dousset, Anne Negre, Robert Salvayre, Pierre Rogalle, Quoc Quan Dang & Louis Douste-Blazy

Authors
  1. Nicole Dousset
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  2. Anne Negre
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  3. Robert Salvayre
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  4. Pierre Rogalle
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  5. Quoc Quan Dang
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  6. Louis Douste-Blazy
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Dousset, N., Negre, A., Salvayre, R. et al. Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase. Lipids 23, 605–608 (1988). https://doi.org/10.1007/BF02535605

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  • DOI: https://doi.org/10.1007/BF02535605

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