Geraniol interferes with membrane functions in strains ofCandida andSaccharomyces

Abstract

Geraniol, an olefinic terpene, was found to inhibit growth ofCandida albicans andSaccharomyces cerevisiae strains. Geraniol was shown to enhance the rate of potassium leakage out of whole cells and also was shown by fluorescence polarization to increaseC. albicans membrane fluidity. Biophysical studies using differential scanning calorimetry, fluorescence polarization and osmotic swelling of phospholipid vesicles demonstrated that geraniol decreased the phase-transition temperature of dipalmitoylphosphatidylcholine vesicles, affected fluidity throughout the bilayer, particularly the central portion of the bilayers, and caused an increase in bilayer permeability to erythritol. Geraniol may have potential use as an antifungal agent.

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Abbreviations

AS:

anthracene stearic acid

DPH:

1,6-diphenyl-1,3,5-hexatriene

DPPC:

dipalmitoylphosphatidylcholine

MIC:

minimum inhibitory concentration

MLV:

multilamellar vesicles

P:

polarization

TMA-DPH:

1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene

YPD:

yeast-complete medium

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Correspondence to Martin Bard.

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Bard, M., Albrecht, M.R., Gupta, N. et al. Geraniol interferes with membrane functions in strains ofCandida andSaccharomyces . Lipids 23, 534–538 (1988). https://doi.org/10.1007/BF02535593

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Keywords

  • Minimal Inhibitory Concentration
  • Ergosterol
  • DPPC
  • Phase Transition Temperature
  • Geraniol