Skip to main content
SpringerLink
Log in

Structure-cytotoxicity studies on alkyl lysophospholipids and some analogs in leukemic blasts of human origin in vitro

  • Published:
Lipids

Abstract

Eleven lipids have been tested for cytotoxic (trypan blue dye exclusion) activity in cells from eight freshly explanted human leukemias in vitro. 4-Aminomethyl-1-[2,3-(di-N-decyloxy)N-propyl]-4-phenylpiperidine (CP-46,665), 1-mercapto-hexadecyl-2-methoxymethyl-rac-glycero-3-phosphocholine (BM 41.440), the 2-acetamide analog of platelet-activating factor (PAF) and 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine (ET-18-OCH3) were found among the most active compounds. 2-Lysophosphatidylcholine (2-LPC) showed the lowest activity. However, in addition there was variation among the results regarding the activity of the 1-octadecyl-rac-glycero-3-phosphocholine (ET-18-OH) and its D- and L-forms, but a significantly higher cytotoxic activity of D-ET-18-OH compared with L-ET-18-OH on the basis of 2-LPC as control after an incubation time of 48 hr. We conclude that with the limited number of structures available, this type of study is not sufficient to yield further information about the mode of the accumulation and toxicity of this type of lipids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ALP:

alkyl lysophospholipid derivative(s)

2-LPC:

2-lysophosphatidylcholine

ANLL:

acute non-lymphoblastic leukemia

ALL:

acute lymphoblastic leukemia

CML/BC:

chronic myeloid leukemia/blast crisis

PAF:

platelet-activating factor

CP-46,665:

4-aminomethyl-1-[2,3-(di-N-decyloxy)N-propyl]-4-phenyl-piperidine

BM 41.440:

rac-1-mercapto-hexadecyl-2-methoxymethyl-rac-glycero-3-phosphocholine

ET-18-OH:

1-O-octadecyl-rac-glycero-3-phosphocholine

ET-18-OCH3 :

1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine

ET-18-H:

1-O-octadecylpropane-3-phosphocholine

ET-12-H:

1-O-dodecylpropanediol-3-phosphocholine

References

  1. Berdel, W.E., Andreesen, R., and Munder, P.G. (1985) inPhospholipids and Cellular Regulation (Kuo, J.F., ed.) Vol. 2, pp. 41–73, CRC Press, Inc., Boca Raton, FL.

    Google Scholar 

  2. Andreesen, R., Modolell, M., Weltzien, H.U., Eibl, H., Common, H.H., Loehr, G.W., and Munder, P.G. (1978)Cancer Res. 38, 3894–3899.

    PubMed  CAS  Google Scholar 

  3. Long, R.C., Small, W.C., Brynes, R.K., Tidwell, T., Goldstein, J.H., and Vogler, W.R. (1983)Cancer Res. 43, 770–775.

    PubMed  CAS  Google Scholar 

  4. Tidwell, T., Guzman, G., and Vogler, W.R. (1981)Blood 57, 794–797.

    PubMed  CAS  Google Scholar 

  5. Berdel, W.E., Fink, U., Egger, B., Reichert, A., Munder, P.G., and Rastetter, J. (1981)J. Natl. Cancer Inst. 66, 813–817.

    PubMed  CAS  Google Scholar 

  6. Runge, M.H., Andreesen, R., Pfleiderer, A., and Munder, P.G. (1980)J. Natl. Cancer Inst. 64, 1301–1306.

    PubMed  CAS  Google Scholar 

  7. Berdel, W.E., Von Hoff, D.D., Unger, C., Schick, H.D., Fink, U., Reichert, A., Eibl, H., and Rastetter, J. (1986)Lipids, 21, 301–304.

    Article  PubMed  CAS  Google Scholar 

  8. Hoffman, D.R., Hajdu, J., and Snyder, F. (1984)Blood 63, 545–552.

    PubMed  CAS  Google Scholar 

  9. Berdel, W.E., Schick, H.D., Fink, U., Reichert, A., Ulm, K., and Rastetter, J. (1985)Cancer Res. 45, 1206–1213.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max Planck Institute for Biophysical Chemistry, Goettingen, Federal Republic of Germany

    Hansjörg Eibl

  2. Department of Medical Statistics and Epidemiology, Technische Universitaet, Munich, Federal Republic of Germany

    Raymonde Busch

  3. Division of Hematology and Oncology, Department of Medicine I, Technische Universität, Ismaninger Str. 22, 8000, Munich 80, Federal Republic of Germany

    Susanne Danhauser, Wolfgang E. Berdel, Hans D. Schick, Michael Fromm, Anneliese Reichert, Ulrich Fink & Johann rastetter

Authors
  1. Susanne Danhauser
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wolfgang E. Berdel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hans D. Schick
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael Fromm
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Anneliese Reichert
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ulrich Fink
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Raymonde Busch
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hansjörg Eibl
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Johann rastetter
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Danhauser, S., Berdel, W.E., Schick, H.D. et al. Structure-cytotoxicity studies on alkyl lysophospholipids and some analogs in leukemic blasts of human origin in vitro. Lipids 22, 911–915 (1987). https://doi.org/10.1007/BF02535553

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02535553

Keywords

Search

Navigation