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Effect of synthetic phospholipids on platelet aggregation and serotonin release

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Lipids

Abstract

1-O-Hexadecyl-2-O-acetyl-sn-glycerol-3-phosphocholine (platelet-activating factor, PAF) is known to stimulate platelet aggregation and serotonin release in concentrations ranging from 10−10–10−5 M. Since a variety of synthetic PAF analogues are potent antineoplastic agents in vitro and in vivo, it was the aim of this study to examine the PAF-like activity of 15 analogues, including 1-O-actadecyl-2-O-methyl-rac-glycero-3-phosphocholine (ET-18-OCH3) and a thioether analogue. In platelet-rich plasma from human blood, platelet aggregation and serotonin release were studied to compare the effects on PAF and the analogues. Platelet function was controlled by testing their response to adenosine diphosphate, arachidonic acid, collagen and epinephrine. Our results show that only PAF was able to induce platelet aggregation and serotonin release in concentrations from 10−9 to 10−5 M, whereas all the tested analogues up to a concentration of 10−3 M failed to induce these effects.

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Abbreviations

PAF:

platelet activating factor

PPP:

platelet-poor plasma

PRP:

platelet-rich plasma

BSA:

bovine serum albumin

ET-18-OCH:

I-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine

BM 41.440:

1-hexadecylmercapto-2-methoxymethyl-rac-glycero-3-phosphocholine

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Authors and Affiliations

  1. Max-Planck-Institute of Biophysical Chemistry, Göttingen, Federal Republic of Germany

    Hansjörg Eibl

  2. Department of Hematology/Oncology Center of Internal Medicine, University of Göttingen, D-34, Göttingen, Federal Republic of Germany

    Ulrike Söling, Gerd A. Nagel & Clemens Unger

Authors
  1. Ulrike Söling
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  2. Hansjörg Eibl
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  3. Gerd A. Nagel
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  4. Clemens Unger
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Söling, U., Eibl, H., Nagel, G.A. et al. Effect of synthetic phospholipids on platelet aggregation and serotonin release. Lipids 22, 868–870 (1987). https://doi.org/10.1007/BF02535546

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  • DOI: https://doi.org/10.1007/BF02535546

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