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Enzymatic acylation of ether and ester lysophospholipids in rat liver microsomes

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Lipids

Abstract

The acylation of lysophospholipids by rat liver acyltransferases was studied. A comparison between ester and ether lysophospholipids as substrates revealed large differences in substrate properties. For instance, oleic acid from oleoyl-CoA and arachidonic acid from arachidonoyl-CoA were not incorporated into 1-O-octadecyl-sn-glycero-3-phosphocholine under experimental conditions that allowed an optimal transfer of oleic acid and arachidonic acid to 1-O-palmitoyl-sn-glycero-3-phosphocholine. However, we observed an acyl-CoA-independent transfer of arachidonic acid from 1-O-stearoyl-2-O-arachidonoyl-sn-glycero-3-phosphoinositol to 1-O-octadecyl-sn-glycero-3-phosphocholine.

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Abbreviations

Lyso-PC:

1-O-palmitoyl-sn-glycero-3-phosphocholine

lyso-PA:

1-O-palmitoyl-sn-glycero-3-phosphate

lyso-PE:

1-O-palmitoyl-sn-glycero-3-phosphoethanolamine

lyso-PS:

1-O-palmitoyl-sn-glycero-3-phosphoserine

lyso-PI:

1-O-palmitoyl-sn-glycero-3-phosphoinositol

DLPC:

1-O-palmitoyl-propandiol-3-phosphocholine

CoA:

coenzyme A

DTNB:

5,5′-dithiobis-2-nitrobenzoic acid

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Authors and Affiliations

  1. Max-Planck-Institut für biophysikalische Chemie, Göttingen, Federal Republic of Germany

    W. Neumüller, E. A. M. Fleer & H. Eibl

  2. Klinikum der Universitat Göttingen, Göttingen, Federal Republic of Germany

    C. Unger

Authors
  1. W. Neumüller
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  2. E. A. M. Fleer
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  3. C. Unger
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  4. H. Eibl
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Neumüller, W., Fleer, E.A.M., Unger, C. et al. Enzymatic acylation of ether and ester lysophospholipids in rat liver microsomes. Lipids 22, 808–812 (1987). https://doi.org/10.1007/BF02535535

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  • DOI: https://doi.org/10.1007/BF02535535

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