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Morphology and fatty acid composition of reticulocytes from phenylhydrazine-treated rats

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Lipids

Abstract

Reticulocytosis was induced in rats by injecting phenylhydrazine, a potent oxidizing agent. Red cell morphology was analyzed by scanning electron microscopy. The majority of red cells from rats given injections of phenylhydrazine were types 2 and 3 echinocytes. Stomatocytes were also observed, but pitted lobular reticulocytes were not detected. Echinocytes have not previously been observed in reticulocyte populations. In the reticulocytes, the relative levels of 16∶1 and 18∶1 were significantly greater than in erythrocytes. These differences in monoenoic acids may be due to the presence of endoplasmic reticulum, the site of desaturase activity in reticulocytes. Of all the fatty acids, the polyunsaturates are the most susceptible to attack during peroxidation. However, the polyunsaturated fatty acid composition of reticulocytes was similar both to that of erythrocytes and to reported values of young erythrocytes isolated by density. Therefore, it is unlikely that lipid peroxidation caused the formation of echinocytes.

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References

  1. van Gastel, C., van der Berg, D., de Gier, J., and van Deenen, L.L.M. (1965) Br. J. Hematol. 11, 193–199.

    Google Scholar 

  2. Phillips, G.R., Dodge J.T., and Howe, C. (1969) Lipids 4, 544–549.

    Article  PubMed  CAS  Google Scholar 

  3. Walker, B., and Yurkowski, M. (1967) Biochem. J. 103, 218–224.

    PubMed  CAS  Google Scholar 

  4. Walter, H., Krob, E.J., and Ascher, G.S. (1978) Br. J. Haematol. 39, 391–398.

    PubMed  CAS  Google Scholar 

  5. Rao, G.A., Siler, K., and Larkin, E.C. (1979) Lipids 14, 30–38.

    Article  PubMed  CAS  Google Scholar 

  6. Bessis, M., Weed, R.I., and Leblond, P.F. (1973) Red Cell Shape: Physiology, Pathology and Ultrastructure, Springer-Verlag, New York, NY.

    Google Scholar 

  7. Mel, H.C., Prenant, M., and Mohandas, N. (1977) Blood 49, 1001–1009.

    PubMed  CAS  Google Scholar 

  8. Coulombel, L., Tchernia, G., and Mohandas, N. (1979) J. Lab. Clin. Med. 94, 467–474.

    PubMed  CAS  Google Scholar 

  9. Rose, H.G., and Oklander, M. (1965) J. Lipid Res. 6, 428–431.

    PubMed  CAS  Google Scholar 

  10. Goldstein, B.D., Rozen, M.G., and Amoruso, M.A. (1979) J. Lab. Clin. Med. 93, 687–694.

    PubMed  CAS  Google Scholar 

  11. Jain, S.K., and Hochstein, P. (1980) Arch. Biochem. Biophys. 201, 683–687.

    Article  PubMed  CAS  Google Scholar 

  12. Tsao, D., Colton, D.G., Chang, J.S., Buck, R.L., Hudson, B.G., and Carraway, K.L. (1977) Br. J. Haematol. 11, 193–199.

    Google Scholar 

  13. Dzandu, J.K., and Johnson, R.M. (1980) J. Biol. Chem. 255, 6382–6386.

    PubMed  CAS  Google Scholar 

  14. Green, G.A., and Kalra, V.K. (1981) J. Biol. Chem. 256, 10565–10571.

    PubMed  CAS  Google Scholar 

  15. Ro, J-Y., Neilan, B., Magee, P.N., Paik, W.K., and Kim, S. (1981) J. Biol. Chem. 256, 10572–10576.

    PubMed  CAS  Google Scholar 

  16. Lubin, B., Chiu, D., Bastacky, J., Roelfsen, B., and Van Deenen, L.L.M. (1981) J. Clin. Invest. 67, 1643–1649.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Hematology Research Laboratory, Veterans Administration Medical Center, 94553, Martinez, CA

    S. C. Goheen, E. C. Larkin & G. Ananda Rao

Authors
  1. S. C. Goheen
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  2. E. C. Larkin
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  3. G. Ananda Rao
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Goheen, S.C., Larkin, E.C. & Rao, G.A. Morphology and fatty acid composition of reticulocytes from phenylhydrazine-treated rats. Lipids 17, 594–597 (1982). https://doi.org/10.1007/BF02535364

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  • DOI: https://doi.org/10.1007/BF02535364

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