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Comparative study of methods for measuring cholesterol in biological fluids

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Lipids

Abstract

A method combining enzymatic and electrochemical detection of cholesterol in biological fluids was compared with conventional detection methods: two chromatographic methods—gas chromatography or high performance liquid chromatography—and two enzymatic methods with colorimetric detection either by kinetic measurement or at the end of the reaction. For serum, enzymatic determination is accurate with both colorimetric detection methods; but for bile, colorimetric detection is difficult to perform due to interference from bile pigments. Enzymatic cholesterol determination, combined with electrochemical detection, is simpler and gives results in good agreement with those of chromatographic methods.

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Abbreviations

GC:

gas chromatography

HPLC:

high performance liquid chromatography

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Author information

Author notes

  1. Franck Cheillan

    Present address: Unite 130, INSERM, 18 Avenue Mozart, 13009, Marseille, France

Authors and Affiliations

  1. Laboratoire de Chimie des Produits Naturels, Faculté des Sciences de St-Jérôme, Avenue Escadrille Normandie-Niémen, 13997, Marseille Cedex 13, France

    Franck Cheillan, Yolande Hamann & Guy Lesgards

  2. Unite 130, INSERM, 18 Avenue Mozart, 13009, Marseille, France

    Huguette Lafont & Elise Termine

Authors
  1. Franck Cheillan
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  2. Huguette Lafont
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  3. Elise Termine
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  4. Yolande Hamann
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  5. Guy Lesgards
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Cheillan, F., Lafont, H., Termine, E. et al. Comparative study of methods for measuring cholesterol in biological fluids. Lipids 24, 224–228 (1989). https://doi.org/10.1007/BF02535239

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  • DOI: https://doi.org/10.1007/BF02535239

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