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Effects of cholesterol oxidation derivatives on cholesterol esterifying and cholesteryl ester hydrolytic enzyme activity of cultured rabbit aortic smooth muscle cells

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Lipids

Abstract

The effects of 5 μg/ml of 25-hydroxycholesterol; cholestane-3β, 5α,6β-triol; and cholesterol on acyl CoA cholesterol acyltransferase, acid cholesteryl ester hydrolase and neutral cholesteryl ester hydrolase was studied in cultured rabbit aortic smooth muscle cells. After 1 hour incubation, 25-hydroxycholesterol resulted in a fourfold stimulation of acyl CoA cholesterol acyltrans-ferase activity. No stimulation by 25-hydroxycholesterol was noted before 15 minutes or after 5 hours of incubation. Neither cholestane-3β,5α,6β-triol nor cholesterol influenced acyl CoA cholesterol acyltransferase activity at any time interval. No significant effects of any of the sterols were noted on acid cholesteryl ester hydrolase or neutral cholesteryl ester hydrolase activity. The imbalance between acyl CoA cholesterol acyl trans-ferase and hydrolase activities induced by 25-hydroxycholesterol could result in cholesteryl ester accumulation by arterial smooth muscle cells, which may be associated with atherosclerosis.

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Abbreviations

ACAT:

acyl CoA cholesterol acyl transferase

ACEH:

acid cholesteryl ester hydrolase

HDL:

high density lipoprotein

HMGCoA:

hydroxy methyl glutaryl coenzyme A

NCEH:

neutral cholesteryl ester hydrolase

SVE:

sucrose containing 1.0 mM EDTA and 1% ethanol

triol:

cholestane-3β,5α,6β-triol

25-OH:

25-hydroxycholesterol

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Authors and Affiliations

  1. Department of Pathology, Harbor-UCLA Medical Center, 90509, Tarrance, CA

    Robert J. Morin & Shi-Kaung Peng

Authors
  1. Robert J. Morin
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  2. Shi-Kaung Peng
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Morin, R.J., Peng, SK. Effects of cholesterol oxidation derivatives on cholesterol esterifying and cholesteryl ester hydrolytic enzyme activity of cultured rabbit aortic smooth muscle cells. Lipids 24, 217–220 (1989). https://doi.org/10.1007/BF02535237

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  • DOI: https://doi.org/10.1007/BF02535237

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