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Microsomal lipid peroxidation: Effect of vitamin E and its functional interaction with phospholipid hydroperoxide glutathione peroxidase

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Lipids

Abstract

The role of vitamin E in the protection against iron dependent lipid peroxidation was studied in rat liver microsomes and Triton-dispersed microsomal lipid micelles. In these systems, an antioxidant effect of vitamin E at a physiological ratio to phospholipids could be observed only in the presence of phospholipid hydroperoxide glutathione peroxidase (PHGPX) and glutathione. The rationale of this cooperation is discussed on the basis of the hydroperoxyl radical scavenging capacity of vitamin E and the reduction of membrane hydroperoxides by PHGPX. The scavenging of lipid hydroperoxyl radicals by vitamin E, although inhibiting propagation of the peroxidative chain, produces lipid hydroperoxides from which ferrous iron generates alkoxyl radicals that react with vitamin E almost as fast as with fatty acids. Therefore, only if membrane hydroperoxides are continuously reduced by this specific peroxidase does the scavenging of hydroperoxyl radicals by vitamin E lead to an effective inhibition of lipid peroxidation.

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Abbreviations

PHGPX:

phospholipid hydroperoxide glutathione peroxidase

L:

lipid carbon centered radical

LOO:

lipid hydroperoxyl radical

LO:

lipid alkoxyl radical

LOOH:

lipid hydroperoxide

MDA:

malondialdehyde

LH:

polyunsaturated fatty acid

ADP:

adenosine diphosphate

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Authors and Affiliations

  1. Department of Biological Chemistry, Padova University, via F. Marzolo 3, 35131, Padova, Italy

    Matilde Maiorino, Mariagrazia Coassin, Antonella Roveri & Fulvio Ursini

Authors
  1. Matilde Maiorino
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  2. Mariagrazia Coassin
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  3. Antonella Roveri
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  4. Fulvio Ursini
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Maiorino, M., Coassin, M., Roveri, A. et al. Microsomal lipid peroxidation: Effect of vitamin E and its functional interaction with phospholipid hydroperoxide glutathione peroxidase. Lipids 24, 721–726 (1989). https://doi.org/10.1007/BF02535211

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  • DOI: https://doi.org/10.1007/BF02535211

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