Abstract
The antioxidant effect of diethyl maleate and reduced glutathione was studied in an in vitro peroxidizing system. Linoleate peroxidation, measured as conjugated diene formation, was not altered by diethyl maleate (2 or 4 mM), whereas the addition of reduced glutathione (1, 2 or 4 mM) elicited a marked and progressive reduction. This effect of glutathione is not modified by diethyl maleate. The inhibition of linoleate peroxidation by glutathione was found concomitantly with a decrease in the concentration of its reduced form and a corresponding increase in glutathione disulfide levels, so that the total equivalents of reduced glutathione in the system remained constant. It is concluded that diethyl maleate does not have antioxidant properties in a peroxidizing system, as found for reduced glutathione.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Videla, L.A., Fernández, V., Ugarte, G., Valenzuela, A., and Villanueva, A. (1980) FEBS Lett. 111, 6–10.
Valenzuela, A., Fernández, N, Fernández, V., Ugarte, G., Videla, L.A., Guerra, R., and Villanueva, A. (1980) FEBS Lett. 111, 11–13.
Chasseaud, L.F. (1976) in Glutathione: Metabolism and Function (Arias, I.M., and Jakoby, W.B., eds.), pp. 77–114, Raven Press, New York, NY.
Juanet, A., Tabak, M., Videla, L.A., and Valenzuela, A. (1981) IRCS Med. Sci. 9, 388.
Hogberg, J., and Kristoferson, A. (1977) Eur. J. Biochem. 74, 77–82.
Wendel, A., Feuerstein, S., and Kong, K.H. (1979) Biochem. Pharmacol. 28, 2051–2055.
Mead, J.F. (1976) in Free Radicals in Biology (Pryor, W.A., ed.) Vol. 1, pp. 51–68, Academic Press, New York, NY.
Hasse, G., and Dunkley, W. (1969) J. Lipid Res. 10, 55–58.
Buege, J.A., and Aust, S.D. (1978) Methods Enzymol. 52, 302–310.
Bernt, F., and Bergmeyer, H.U. (1974) in Methods of Enzymatic Analysis (Bergmeyer, H.U., ed.) Vol. 4, pp. 1643–1647, Academic Press, New York, NY.
Chance, B., Sies, H., and Boveris, A. (1979) Physiol. Rev. 59, 527–605.
Sies, H., Wahllander, A., Waydhas, C., Soboll, S., and Haberle, D. (1980) Adv. Enz. Reg. 18, 303–320.
Meister, A. (1975) in Metabolic Pathways (Greenberg, D.M., ed.) Vol. 7, pp. 101–188, Academic Press, New York, NY.
Kosower, N.S., and Kosower, E.M. (1979) Int. Rev. Cytol. 54, 109–160.
Chasseaud, L.F. (1973) Drug Metab. Rev. 2, 185–220.
Author information
Authors and Affiliations
About this article
Cite this article
Fernandez, N., Valenzuela, A., Fernandez, V. et al. Effect of diethyl maleate and glutathione on linoleate peroxidation. Lipids 17, 393–395 (1982). https://doi.org/10.1007/BF02535200
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02535200