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Eicosapentaenoic and arachidonic acid: Comparison of metabolism and activity in murine epidermal cells

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Lipids

Abstract

The biological activity, including metabolism and modulation of ornithine decarboxylase activity and DNA synthesis, of arachidonic acid (AA) and eicosapentaenoic acid (EPA) were compared in epidermal cells from SENCAR mice. Radiolabelled AA and EPA were found to be similarly incorporated into and released from membrane phospholipids of unstimulated cultures. However, when cells were stimulated with the tumor promoter 12-0-tetradecanoylphorbol-13-acetate (TPA), the release of AA was significantly higher than the release of EPA. The extent of metabolism of AA and EPA to prostaglandins was determined in both freeze-thawed cell preparations and in viable cultured cells. In the freeze-thawed preparations, use of AA as a substrate resulted in significantly more PGF than when EPA was used as the substrate. However, more PGE3 was formed than PGE2. PGD levels were the same for either fatty acid precursor. Prostaglandin production was also determined in viable cultured cells since other influences such as phospholipase A2 activity can modify prostaglandin production. Control cultures prelabelled with either AA or EPA produced similar amounts of the respective PGF, PGE, and PGD. However, TPA-stimulated cultures produced significantly higher amounts of each prostaglandin in cultures prelabelled with AA compared to cells prelabelled with EPA. HETE or HEPE production was the same both for cultured cells prelabelled with AA or EPA and for homogenates from uncultured cells incubated directly with the radiolabelled fatty acids. TPA-induced ornithine decarboxylase (ODC) was significantly higher in AA-treated cultures compared to EPA-treated cultures. AA supports DNA synthesis to a greater extent than EPA, either alone or in the presence of TPA. These findings suggest that AA and EPA do not have equivalent biological activity in mouse epidermal cells.

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Abbreviations

AA:

arachidonic acid

EPA:

eicosapentaenoic acid

TPA:

12-0-tetradecanoylphorbol-13-acetate

ODC:

ornithine decarboxylase

HETE:

hydroxyeicosatetraenoic acid

HEPE:

hydroxyeicosapentaenoic acid

PG:

prostaglandin

TLC:

thin layer chromatography

References

  1. Cohen, L.A., Thompson, D.O., Maerura, Y., Choi, K., Blank, M.E., and Rose, D.P. (1986)J. Natl. Cancer Inst. 77, 33–42.

    PubMed  CAS  Google Scholar 

  2. Carroll, K.K., and Khor, H.T. (1971)Lipids 6, 415–420.

    Article  PubMed  CAS  Google Scholar 

  3. Ip, C., Carter, C.A., Ip, M.M. (1985)Cancer Res. 45, 1997–2001.

    PubMed  CAS  Google Scholar 

  4. Chan, P., Ferguson, K.A., and Dao, T.L. (1983)Cancer Res. 43, 1079–1083.

    PubMed  CAS  Google Scholar 

  5. Jurkowski, J.J., and Cave, W.T. (1985)J. Natl. Cancer Inst. 74, 1145–1150.

    PubMed  CAS  Google Scholar 

  6. Gabor, H., and Abraham, S. (1986)J. Natl. Cancer Inst. 76, 1223–1229.

    PubMed  CAS  Google Scholar 

  7. Karmali, R.A., Marsh, J., and Fuchs, C. (1984)J. Natl. Cancer Inst. 73, 457–461.

    PubMed  CAS  Google Scholar 

  8. O'Connor, T.P., Roebuck, B.D., Peterson, F., and Campbell, T.C. (1985)J. Natl. Cancer Inst. 75, 959–962.

    PubMed  Google Scholar 

  9. Cohen, L.A., Thompson, D.O., Choi, K., Karmali, R.A., and Rose, D.P. (1986)J. Natl. Cancer Inst. 77, 43–51.

    PubMed  CAS  Google Scholar 

  10. Furstenberger, G., Gross, M., and Marks, F. (1984) inIcosanoids and Cancer (H. Thaler-Dao, A. Crastes de Paulet and R. Paoletti, eds.) pp. 91–100, Raven Press, Inc., New York.

    Google Scholar 

  11. Verma, A.K., Ashendel, C.L., and Boutwell, R.K. (1980)Cancer Res. 40, 308–315.

    PubMed  CAS  Google Scholar 

  12. Fischer, S.M. (1985) inArachidonic Acid Metabolism and Tumor Promotion (S.M. Fischer and T.J. Slaga, eds.) pp. 21–47, Martinus Nijhoff Publ., Boston.

    Google Scholar 

  13. Brune, K., Kalin, H., Schmidt, R., and Hecker, E. (1978)Cancer Lett. 4, 333–342.

    Article  PubMed  CAS  Google Scholar 

  14. Bresnick, E., Neunier, P., and Lamden, M. (1979)Cancer Lett. 7, 121–125.

    Article  PubMed  CAS  Google Scholar 

  15. Fischer, S.M., Gleason, G.L., Mills, G.D., Slaga, T.J. (1980)Cancer Lett. 10, 343–350.

    Article  PubMed  CAS  Google Scholar 

  16. Verma, A.K., and Boutwell, R.K. (1977)Biochem. Biophys. Res. Comm. 79, 1160–1166.

    PubMed  CAS  Google Scholar 

  17. Slaga, T.J., Fischer, S.M., Weeks, L.E., and Klein-Szanto, A.J.P. (1980) inBiochemistry of Normal and Abnormal Epidermal Differentiation (I.A. Bernstein and M. Seiji, eds.) pp. 193–218, S. Karger, New York.

    Google Scholar 

  18. Croft, K.D., Beilin, L.J., Legge, F.M., and Vandongen, R. (1987)Lipids 22, 647–650.

    CAS  Google Scholar 

  19. Swanson, J.E., Black, J.M., and Kinsella, J.E. (1987)J. Nutr. 117, 824–832.

    PubMed  CAS  Google Scholar 

  20. Morita, I., Saito, Y., Chang, W.C., and Murota, S. (1983)Lipids 18, 42–49.

    Article  PubMed  CAS  Google Scholar 

  21. Weaver, B.J., and Holub, B.J. (1985)Lipids 20, 773–777.

    Article  PubMed  CAS  Google Scholar 

  22. Needleman, P., Whitaker, M.O., Wyche, A., Watters, K., Sprecher, H., and Raz, A. (1980)Prostaglandins 19, 165–181.

    Article  PubMed  CAS  Google Scholar 

  23. Seiss, W., Roth, P., Scherer, B., Kurzmann, I., Bohlig, B., and Weber, P.C. (1980)Lancet I, 441–444.

    Article  Google Scholar 

  24. Fischer, S.M., O'Connell, J.F., Conti, C.J., Tacker, K.C., Fries, J.W., Patrick, K.E., Adams, L.M., Slaga, T.J. (1987)Carcinogenesis 8, 421–424.

    PubMed  CAS  Google Scholar 

  25. Yuspa, S.H., and Harris, C.C. (1974)Exptl. Cell. Res. 86, 95–105.

    Article  PubMed  CAS  Google Scholar 

  26. Morris, R.J., Tacker, K.C., Baldwin, J.K., Fischer, S.M., and Slaga, T.J., (1987)Cancer Lett. 34, 297–304.

    Article  PubMed  CAS  Google Scholar 

  27. Lepage, M. (1964)J. Lipid Res. 5, 587.

    PubMed  Google Scholar 

  28. Flower, R.J., and Blackwell, G.J. (1976)Biochem. Pharm. 25, 285–291.

    Article  PubMed  CAS  Google Scholar 

  29. Fischer, S.M., Baldwin, J.K., Jasheway, D.W., Patrick, K.E., and Cameron, G.S. (1988)Cancer Res. 48, 658–664.

    PubMed  CAS  Google Scholar 

  30. Gschwendt, M., Kittstein, W., Horn, F., and Marks, F. (1985)Biochem. Biophys. Res. Comm. 126, 327–332.

    Article  PubMed  CAS  Google Scholar 

  31. Weeks, C.E., and Slaga, T.J. (1979)Biochem. Biophys. Res. Comm. 91, 1488–1496.

    Article  PubMed  CAS  Google Scholar 

  32. Schmidt, G., and Thannhauser, S.J. (1945)J. Biol. Chem. 161, 83–89.

    CAS  Google Scholar 

  33. Burton, K. (1968)Meth. Enzymol. 12, 163–166.

    Article  CAS  Google Scholar 

  34. Levine, L., and Hassid, R.A. (1977)Biochem. Biophys. Res. Commun. 79:477–484.

    Article  PubMed  CAS  Google Scholar 

  35. Lands, W.E.M., and Byrnes, M.J. (1982)Prog. Lipid Res. 20, 287–290.

    Article  Google Scholar 

  36. Magrum, L.J., and Johnston, P.V. (1985)Biochim. Biophys. Acta 836, 354–360.

    PubMed  CAS  Google Scholar 

  37. Craig-Schmidt, M.C., Faircloth, S.A., and Weete, J.D. (1987)J. Nutr. 117, 1197–1206.

    PubMed  CAS  Google Scholar 

  38. Pace-Asciak, C., and Wolfe, L.S. (1968)Biochim. Biophys. Acta 152, 784–787.

    PubMed  CAS  Google Scholar 

  39. Ziboh, V.A., Vanderhoek, J.Y., and Lands, W.E.M. (1974)Prostaglandins 5, 233–240.

    Article  PubMed  CAS  Google Scholar 

  40. Culp, B.R., Titus, B.E., and Lands, W.E.M. (1979)Prostaglandins Med. 3, 269–278.

    Article  PubMed  CAS  Google Scholar 

  41. Mohrhauer, H., and Holman, R.T. (1963)J. Nutr. 81, 67–74.

    PubMed  CAS  Google Scholar 

  42. Hamberg, M., Svenson, J., and Samuelsson, B. (1974)Proc. Natl. Acad. Sci. 71, 3824–3828.

    Article  PubMed  CAS  Google Scholar 

  43. Vanderhoek, J.Y., Bryant, R.W., and Bailey, J.M. (1980)J. Biol. Chem. 255, 10064–10065.

    PubMed  CAS  Google Scholar 

  44. Hammarström, S., Hamberg, M., Samuelsson, B., Duell, E.A., Staviski, M., and Voorhees, J.J. (1975)Proc. Natl. Acad. Sci. 72, 5130–5134.

    Article  PubMed  Google Scholar 

  45. Kato, R., Nakadate, T., Yamamoto, S., and Sugimara, T. (1983)Carcinogenesis 4, 1301–1305.

    PubMed  CAS  Google Scholar 

  46. Nakadate, T., Yamamoto, S., and Ishii, M., and Kato, R. (1982)Cancer Res. 42, 2841–2845.

    PubMed  CAS  Google Scholar 

  47. Nakadate, T., Yamamoto, S., and Ishii, M., and Kato, R. (1982)Carcinogenesis 3, 1411–1414.

    PubMed  CAS  Google Scholar 

  48. Fischer, S.M., Fürstenberger, G., Marks, F., and Slaga, T.J. (1987)Cancer Res. 47, 3174–3179.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Science Park-Research Division, University of Texas M.D. Anderson Cancer Center, 78957, Smithville, TX

    Kelly E. Patrick & Susan M. Fischer

  2. Dvision of Graduate Nutrition, University of Texas at Austin, 78712, Austin, TX

    Martha A. Belury & Mary Locniskar

Authors
  1. Martha A. Belury
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  2. Kelly E. Patrick
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  3. Mary Locniskar
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  4. Susan M. Fischer
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Belury, M.A., Patrick, K.E., Locniskar, M. et al. Eicosapentaenoic and arachidonic acid: Comparison of metabolism and activity in murine epidermal cells. Lipids 24, 423–429 (1989). https://doi.org/10.1007/BF02535150

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  • DOI: https://doi.org/10.1007/BF02535150

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