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Δ6 and Δ5 desaturase activities in liver from obese zucker rats at different ages

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Lipids

Abstract

Δ6 Desaturation of linoleic acid (18∶2 n−6) and Δ5 desaturation of dihomo-γ-linolenic acid (20∶3 n−6) were measured in liver microsomes from genetically obese Zucker rats (fa/fa) and from their lean littermates (Fa/−). Both groups were fed a balanced commercial diet. The rats were 6, 9 and 12 weeks old, which corresponded to stages in their active growth period. The content of total fatty acids and n−6 polyunsaturated fatty acids in whole liver and liver microsomes was also determined in order to ascertain how the desaturase activities measuredin vitro reflected regulation of essential fatty acid metabolismin vivo. Contrary to values obtained for Δ6 desaturation, Δ5 desaturation at nonsaturating substrate levels were lower in obese rats than in lean controls. In contrast, at saturating substrate level, the maximal Δ5 desaturase activities were the same in both phenotypes and they increased with age. Study of Δ5 desaturation kinetics (1/V vs 1/S) showed that Vm did not differ between 12-week-old obese and lean rats, whereas KM in obese rats was much lower than in controls, expressing the very low affinity of the enzyme for the substrate in obese animals. The fatty acid composition of liver lipids reflected the results of desaturase activitiesin vitro. In particular, the ratios 20∶4 n−6/20∶3 n−6 were lower in obese rats than in lean rats, which can be explained by the lower conversion of 20∶3 n−6 into 20∶4 n−6 by Δ5 desaturation. However, the total amount of 20∶4 n−6 in the whole liver did not differ between phenotypes, whatever their age. This work presents evidence for a relationship between the changes in fatty acid compositional data in hepatic total lipids, total lipids of liver microsomes and modifications of fatty acid desaturase activities in the genetically obese Zucker rat.

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References

  1. Bray, G.A. (1977)Fed. Proc., Fed. Am. Soc. Exp. Biol. 36, 148–153.

    CAS  Google Scholar 

  2. Bach, A., Schirardin, H., Bauer M., Schaeffer, A., and Weryha, A. (1981)Lipids 16, 841–848.

    Article  PubMed  CAS  Google Scholar 

  3. Zucker, L.M., and Antoniades, H.N. (1972)Endocrinology 90, 1320–1330.

    Article  PubMed  CAS  Google Scholar 

  4. Lemonnier, D., Aubert, R., Suquet, J.P., and Rosselin, G. (1974)Diabetologia 10, 97–101.

    Article  Google Scholar 

  5. Martin, R.J. (1974)Life Sci. 14, 1447–1453.

    Article  PubMed  CAS  Google Scholar 

  6. Godbole V., and York, D.A. (1978)Diabetologia 14, 191–197.

    Article  PubMed  CAS  Google Scholar 

  7. Wahle, K.W.J. (1974)Comp. Biochem. Physiol. 48 B, 565–574.

    Google Scholar 

  8. Wahle, K.W.J., and Radcliffe, J.D. (1977)Lipids 12, 135–139.

    Article  PubMed  CAS  Google Scholar 

  9. Weekes, T.E.C., Wahle, K.W.J., and Lebaijuri, M.B. (1986)Lipids 21, 220–225.

    Article  PubMed  CAS  Google Scholar 

  10. Chanussot, F., Karsenty, C., and Debry, G. (1983)Repr. Nutr. Develop 23, 717–725.

    CAS  Google Scholar 

  11. Blond, J.P., Precigou, P., and Bézard, J. (1988)Arch. Int. Physiol. Biochim. 96, 41–49.

    PubMed  CAS  Google Scholar 

  12. Pande, S.V., and Mead, J.F. (1968)J. Biol. Chem. 243, 352–361.

    PubMed  CAS  Google Scholar 

  13. Suzue, G., and Marcel, Y.L. (1972)J. Biol. Chem. 247, 6781–6783.

    PubMed  CAS  Google Scholar 

  14. Brenner, R.R. (1971)Lipids 6, 567–575.

    Article  PubMed  CAS  Google Scholar 

  15. Sprecher, H. (1977)Adv. Exptl. Med. Biol. 83, 33–50.

    Google Scholar 

  16. Naughton, J.M. (1981)Int. J. Biochem. 13, 21–32.

    Article  PubMed  CAS  Google Scholar 

  17. Lee, C.J., and Sprecher, H. (1971)Biochim. Biophys. Acta 248, 180–185.

    PubMed  CAS  Google Scholar 

  18. Nervi, A.M., Catala, A., Brenner, R.R., and Peluffo, R.O. (1975)Lipids 10, 348–352.

    Article  PubMed  CAS  Google Scholar 

  19. Jeffcoat, R., and James, A.T. (1977)Lipids 12, 469–474.

    PubMed  CAS  Google Scholar 

  20. Kurata, N., and Privett, O.S. (1980)Lipids 15, 953–961.

    Article  Google Scholar 

  21. De Gomez Dumm, I.N.T., De Alaniz, M.J.T., and Brenner, R.R. (1983)Lipids 18, 781–788.

    PubMed  Google Scholar 

  22. Blond, J.P., Ratieuville, P., and Bézard, J. (1985)Cah. Nutr. Diet. 20, 109–111.

    CAS  Google Scholar 

  23. Faas, F.H., and Carter, W.J. (1980)Lipids 15, 953–961.

    Article  CAS  Google Scholar 

  24. Brenner, R.R., Peluffo, R.O., Mercuri, O., and Restelli, M.A. (1968)Am. J. Physiol. 215, 63–70.

    PubMed  CAS  Google Scholar 

  25. Poisson, J.P. (1985)Enzyme 34, 1–14.

    PubMed  CAS  Google Scholar 

  26. Blond, J.P., Ratieuville, P., and Bézard, J. (1986)Reprod. Nutr. Develop. 26, 77–84.

    CAS  Google Scholar 

  27. Smith, P.A., and Kaplan, M.L. (1980)Int. J. Biochem. 11, 217–228.

    Article  PubMed  CAS  Google Scholar 

  28. Layne, E. (1957) inMethods in Enzymology (Colowick, S.P., and Kaplan, N.O.) Vol. 3, pp. 447–454, Academic Press, New York.

    Google Scholar 

  29. Kirstein, D., Hoy, C.E., and Hölmer, G. (1983)Br. J. Nutr. 50, 749–756.

    Article  PubMed  CAS  Google Scholar 

  30. Jeffcoat, R. (1979) inEssays in Biochemistry 15, 1–36.

  31. Bézard, J., Boucrot, P., and Clément, G. (1964)J. Chromatogr. 14, 368–377.

    Article  Google Scholar 

  32. Delsal, J.L. (1944)Bull. Soc. Chim. Biol. 26, 99–105.

    CAS  Google Scholar 

  33. Hirsch, J., and Ahrens, E.H. Jr. (1958)J. Biol. Chem. 233, 311–320.

    PubMed  CAS  Google Scholar 

  34. Duncan, D.B. (1955)Biometrics 11, 1–42.

    Article  Google Scholar 

  35. Wahle, K.W.J., Duncan, A.M., and Coutts,L. (1984)Proc. Nutr. Soc. 43, 97A

    Google Scholar 

  36. Pugh, E.L., and Kates, M. (1977)J. Biol. Chem. 252, 68–73.

    PubMed  CAS  Google Scholar 

  37. De Gomez-Dumm, I.N.T., De Alaniz, M.T., and Brenner, R.R. (1977)Adv. Exp. Med. Biol. 83, 609–616.

    PubMed  Google Scholar 

  38. Faas, F.H., and Carter, W.J. (1982)Biochem. J. 207, 29–35.

    PubMed  CAS  Google Scholar 

  39. Clouet, P., Henninger, C., Pascal, M., and Bézard, J. (1985)FEBS Lett. 182 331–334.

    Article  PubMed  CAS  Google Scholar 

  40. Clouet, P., Henninger, C., and Bézard, J. (1986)Biochem. J. 239, 103–108.

    PubMed  CAS  Google Scholar 

  41. Landgraf-Leurs, M.M.C., Loy, A., Christea, C., Weber, P.C., Siess, W., Herberg, L.L., and Landgraf, R. (1981)Prostaglandins 22, 521–536.

    Article  PubMed  CAS  Google Scholar 

  42. Cunnane, S.C., Manku, M.S., and Horrobin, D.F. (1985)Br. J. Nutr. 53, 441–448.

    Article  PubMed  CAS  Google Scholar 

  43. Hughes, S., and York, D.A. (1985)Biochem. J. 225, 307–313.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculté des Sciences Mirande, Laboratoire de Physiologie Animale et de la Nutrition, U.A. C.N.R.S. 273, B.P. 138, 21004, Dijon Cedex, France

    Jean-Paul Blond, Cherifa Henchiri & Jean Bézard

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  1. Jean-Paul Blond
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  2. Cherifa Henchiri
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  3. Jean Bézard
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Blond, JP., Henchiri, C. & Bézard, J. Δ6 and Δ5 desaturase activities in liver from obese zucker rats at different ages. Lipids 24, 389–395 (1989). https://doi.org/10.1007/BF02535146

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  • DOI: https://doi.org/10.1007/BF02535146

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