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Constituents of human meconium: II. Identification of steroidal acids with 21 and 22 carbon atoms

  • Symposium: Sterols Held at the AOCS Annual Meeting in New Orleans, LA, May 17–21, 1981
  • Published:
Lipids

Abstract

Monohydroxylated acid fraction isolated from human meconium was found to contain, in addition to C20 and C24 acids identified previously, three C22 bile acids-(20S)-3α-hydroxy-23,24-bisnor-5β-cholan-22-oic, (20S)- and (20R)-3β-hydroxy-23,24-bisnor-chol-5-en-22-oic, and one C21 acid-3β-hydroxypregn-5-en-21-oic. These compounds were identified by capillary gas chromatography-mass spectrometry and by comparison with standards. It is postulated that these C22 acids, as well as the two monohydroxylated C24 bile acids (lithocholic and 3β-hydroxychol-5-enoic) are produced in the maternal intestine by microbial flora and transferred to the fetus through the placenta.

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Internal Medicine and Department of Pediatrics, University of Texas Medical School at Houston, PO Box 20708, 77025, Houston, TX

    Jan St. Pyrek, Roman Sterzycki, Roger Lester & Eugene Adcock

Authors
  1. Jan St. Pyrek
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  2. Roman Sterzycki
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  3. Roger Lester
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  4. Eugene Adcock
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Additional information

Part I: J. St. Pyrek, R. Lester, E. Adcock, and A.T. Sanghvi, manuscript submitted for publication. The “reductive-extraction” of meconium with ethanol containing large excess of sodium borohydride excluded the artifactual formation of C20 acids as a result of the degradation of pregnane derivatives with hydroxyketone and hydroxyaldehyde side chains.

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Pyrek, J.S., Sterzycki, R., Lester, R. et al. Constituents of human meconium: II. Identification of steroidal acids with 21 and 22 carbon atoms. Lipids 17, 241–249 (1982). https://doi.org/10.1007/BF02535111

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  • DOI: https://doi.org/10.1007/BF02535111

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