Abstract
Chronic treatment of adult male rats with ethanol liquid diets resulted in alterations in phospholipid and cholesterol contents as well as the acyl composition of phosphatidylethanolamine (PE), phosphatidylinositol (PI)-phosphatidylserine (PS) mixture, and phosphatidylcholine (PC) of isolated hepatocytes. The influence of ethanol on these lipids was largely dependent on the proportion of dietary fat. Phospholipid and total cholesterol contents were elevated 23 and 27%, respectively, by ethanol when offered in a low-fat diet (5% corn oil). Only the percentage of arachidonic acid from PI-PS was significantly reduced in the low-fat ethanol group. Exposure to a high-fat (34% corn oil) diet in the presence of ethanol for 4–5 weeks resulted in a significant decrease in arachidonate/linoleate ratios of hepatic PE, PS-PI and PC, while total phospholipid content remained constant. In the high-fat, ethanol-treated group, hepatic cholesterol content was increased 2-fold. These results suggest that the level of dietary fat plays an important role in determining the effects of chronic ethanol consumption on hepatic cholesterol content and phospholipid acyl composition.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hill, M.W., and Bangham, A.D. (1975) Adv. Exp. Med. Biol. 59, 1–9.
Chin, J.H., and Goldstein, D.B. (1977) Science 196, 684–685.
Chin, J.H., Parsons, L.M., and Goldstein, D.B. (1978) Biochim. Biophys. Acta 513, 358–363.
Sun, G.Y., and Sun, A.Y. (1978) Res. Commun. Chem. Pathol. Pharmacol. 21, 355–358.
Starich, G.H., and Reitz, R.C. (1979) Toxicol. Lett. 4, 1–5.
Thompson, J.A., and Reitz, R.C. (1978) Lipids 13, 540–550.
Miceli, J.N. (1973) Lipids 8, 722–727.
Lefevre, A.F., DeCarli, L.M., and Lieber, C.S. (1972) J. Lipid Res. 13, 48–55.
Mendenhall, C.L., Bradford, R.H., and Furman, R.H. (1969) Biochim. Biophys. Acta 187, 501–509.
Fallon, H.J., Gertman, P.M., and Kemp, E.J. (1969) Biochim. Biophys. Acta 187, 94–104.
Joly, J.G., and Hetu, C. (1977) Can. J. Physiol. Pharmacol. 55, 34–41.
Hayes, J.S., and Brendel, K. (1976) Biochem. Pharmacol. 25, 1495–1500.
Smith, T.L., Eichberg, J., and Hauser, G. (1979) Life Sci. 24, 2179–2184.
Bartlett, G.R. (1959) J. Biol. Chem. 234, 466–468.
Rudel, L.L., and Morris, M.D. (1973) J. Lipid Res. 14, 364–366.
Waring, A.J., Rottenberg, H., Ohnishi, T., and Rubin, E. (1981) Proc. Natl. Acad. Sci. USA 78, 2582–2586.
Forsander, C.A., and Lindros, K.D. (1967) Acta Chem. Scand. 21, 2568–2575.
Lieber, C.S., Jones, D.P., and DeCarli, L.M. (1965) J. Clin. Invest. 44, 1009–1021.
Goheen, S.C., Larkin, E.C., Manix, M., and Rao, G.A. (1980) Lipids 15, 328–336.
Author information
Authors and Affiliations
About this article
Cite this article
Smith, T.L., Vickers, A.E., Brendel, K. et al. Effects of ethanol diets on cholesterol content and phospholipid acyl composition of rat hepatocytes. Lipids 17, 124–128 (1982). https://doi.org/10.1007/BF02535091
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02535091