Skip to main content
SpringerLink
Log in

Effects of omega-3 fatty acids on vascular smooth muscle cells: Reduction in arachidonic acid incorporation into inositol phospholipids

  • Published:
Lipids

Abstract

A rapid increase in arachidonic acid incorporation into phosphatidylinositol (PI) occurred following exposure of cultured porcine pulmonary artery smooth muscle cells to calcium ionophore A23187. This response was specific for PI and phosphatidic acid; none of the other phosphoglycerides showed any increase in arachidonic acid incorporation. The incorporation of [3H]inositol also was increased, indicating that complete synthesis of PI rather than only fatty acylation occurred in response to the ionophore. The presence of omega-3 fatty acids, especially eicosapentaenoic acid (EPA), reduced arachidonic acid but not inositol incorporation into PI. Stimulated incorporation of EPA also occurred under these conditions, suggesting that EPA replaces arachidonic acid in the newly synthesized pool of PI. Although much less arachidonic acid was incorporated into the polyphosphoinositides following exposure to the ionophore, arachidonic acid incorporation into these phosphorylated derivatives also decreased when EPA was present. These findings suggest that when omega-3 fatty acids are available, less arachidonic acid is channeled into the inositol phospholipids of activated smooth muscle cells because of replacement by EPA. This may represent a mechanism whereby omega-3 fatty acids, especially EPA, can accumulate in the metabolically active pools of inositol phospholipids and thereby possibly influence the properties or responsiveness of vascular smooth muscle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EPA:

eicosapentaenoic acid

DHA or 22∶6:

docosahexaenoic acid

PGI2 :

prostacyclin (prostaglandin I2)

18∶3:

linolenic acid

BSA:

bovine serum albumin

PA:

phosphatidic acid

TLC:

thin layer chromatography

GLC:

gas-liquid chromatography

HPLC:

high performance liquid chromatography

PC:

choline phosphoglycerides

PE:

ethanolamine phosphoglycerides

PS:

serine phosphoglycerides

PI:

inositol phosphoglycerides

PGE2 :

prostaglandin E2

HETE:

hydroxyeicosatetraenoic acid

16∶0:

palmitic acid

18∶1:

oleic acid

PIP:

phosphatidylinositol-4-phosphate

PIP2 :

phosphatidylinositol-4,5-bisphosphate

References

  1. Dyerberg, J., and Bang, H.O. (1979)Lancet ii, 433–435.

    Article  Google Scholar 

  2. Kromhout, D., Bosschieter, E.B., and Coulander, C.L. (1985)N. Engl. J. Med. 312, 1205–1209.

    Article  PubMed  CAS  Google Scholar 

  3. Phillipson, B.E., Rothcock, D.W., Connor, W.C., Harris, W.S., and Illingworth, D.R. (1985)N. Engl. J. Med. 312, 1210–1216.

    Article  PubMed  CAS  Google Scholar 

  4. Lee, T.H., Hoover, R.L., Williams, J.D., Sperling, R.I., Revalese, J., Spur, B.W., Robinson, D.R., Corey, E.J., Lewis, R.A., and Austin, K.F. (1985)N. Engl. J. Med. 312, 1217–1224.

    Article  PubMed  CAS  Google Scholar 

  5. Whitaker, M.O., Wyche, A., Fitzpatrick, F., Sprecher, H., and Needleman, P. (1979)Proc. Natl. Acad. Sci. USA 76, 5919–5923.

    Article  PubMed  CAS  Google Scholar 

  6. Prescott, S.M. (1984)J. Biol. Chem. 259, 7615–7621.

    PubMed  CAS  Google Scholar 

  7. Illingworth, D.R., Harris, W.S., and Connor, W.E. (1984)Arteriosclerosis 4, 270–275.

    PubMed  CAS  Google Scholar 

  8. Spector, A.A., Kaduce, T.L., Hoak, J.C., and Czervionke, R.L. (1983)Arteriosclerosis 3, 332–337.

    Google Scholar 

  9. Hadjiagapiou, C., Kaduce, T.L., and Spector, A.A. (1986)Biochim. Biophys. Acta 1875, 369–381.

    Google Scholar 

  10. Hadjiagapiou, C., and Spector, A.A. (1987)Arch. Biochem. Biophys. 253, 1–12.

    Article  PubMed  CAS  Google Scholar 

  11. Morisaki, N., Kanazaki, T., Fujiyama, Y., Oswa, I., Shirai, K., Matsuoka, N., Saito, Y., and Yoshida, S. (1985)J. Lipid Res. 26, 930–939.

    PubMed  CAS  Google Scholar 

  12. Morita, I., Saito, Y., Chang, W.C., and Murota, S. (1983)Lipids 18, 42–49.

    Article  PubMed  CAS  Google Scholar 

  13. Ross, R. (1986)New Engl. J. Med. 314, 488–500.

    Article  PubMed  CAS  Google Scholar 

  14. Berridge, M.J., and Irvine, R.F. (1984)Nature 312, 315–321.

    Article  PubMed  CAS  Google Scholar 

  15. Stoll, L.L., and Spector, A.A. (1987)J. Cell. Physiol. 133, 103–110.

    Article  PubMed  CAS  Google Scholar 

  16. Ross, R. (1971)J. Cell Biol. 50, 172–186.

    Article  PubMed  CAS  Google Scholar 

  17. Strauch, A.R., and Rubenstein, P.A. (1984)J. Biol. Chem. 259, 3152–3159.

    PubMed  CAS  Google Scholar 

  18. Folch, J.M., Lees, M., and Sloan-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  19. Schacht, J. (1981)Methods Enzymol. 72, 626–631.

    PubMed  CAS  Google Scholar 

  20. Jolles, J., Zwiers, H., Dekker, A., Wirtz, K.W.A., and Gispen, W.H. (1981)Biochem. J. 194, 283–291.

    PubMed  CAS  Google Scholar 

  21. Morrison, W.R., and Smith, M.L. (1964)J. Lipid Res. 5, 600–608.

    PubMed  CAS  Google Scholar 

  22. Lapetina, E.G., and Cuatrecasas, P. (1979)Biochim. Biophys. Acta 573, 394–402.

    PubMed  CAS  Google Scholar 

  23. Eling, T., Tainer, B., Ally, A., and Warnock, R. (1982)Methods Enzymol. 86, 511–517.

    Article  PubMed  CAS  Google Scholar 

  24. Figard, P.H., Hejlik, D.P., Kaduce, T.L., Stoll, L.L., and Spector, A.A. (1986)J. Lipid Res. 27, 771–780.

    PubMed  CAS  Google Scholar 

  25. Holub, C.J., and Kuksis, S. (1978)Adv. Lipid Res. 16, 1–125.

    PubMed  CAS  Google Scholar 

  26. Hong, S.L., McLaughlin, N.J., Tzeng, C., and Patton, G. (1985)Thrombosis Res. 38, 110.

    Google Scholar 

  27. Hokin, K.E. (1985)Ann. Rev. Biochem. 54, 205–235.

    Article  PubMed  CAS  Google Scholar 

  28. Mahadevappa, V.G., and Holub, B.J. (1983)J. Biol. Chem. 258, 5337–5339.

    PubMed  CAS  Google Scholar 

  29. Dudley, D.T., and Spector, A.A. (1986)Biochem. J. 236, 235–242.

    PubMed  CAS  Google Scholar 

  30. Luthra, M.G., and Sheltaway, A. (1976)J. Neurochem. 27, 1503–1511.

    Article  CAS  Google Scholar 

  31. Sugiura, T., and Waku, K. (1984)Biochim. Biophys. Acta 796, 190–198.

    PubMed  CAS  Google Scholar 

  32. Sasaki, T., and Hasegawa-Sassaki, H. (1985)Biochim. Biophys. Acta 833, 316–322.

    PubMed  CAS  Google Scholar 

  33. Lapetina, E.G., Reep, B., Ganong, B.R., and Bell, R.M. (1985)J. Biol. Chem. 260, 1358–1361.

    PubMed  CAS  Google Scholar 

  34. Davis, R.J., Ganong, B.R., Bell, R.M., and Czech, M.P. (1985)J. Biol. Chem. 260, 5315–5322.

    PubMed  CAS  Google Scholar 

  35. Holub, B.J., and Piekarski, J. (1978)J. Neurochem. 31, 903–908.

    Article  PubMed  CAS  Google Scholar 

  36. Holub, B.J., and Piekarski, J. (1979)Lipids 14, 309–311.

    Article  PubMed  CAS  Google Scholar 

  37. Bell, R.L., and Majerus, P.W. (1980)J. Biol. Chem. 255, 1790–1792.

    PubMed  CAS  Google Scholar 

  38. Hong, S.L., and Deykin, D. (1981)J. Biol. Chem. 256, 5215–5219.

    PubMed  CAS  Google Scholar 

  39. Marshall, P.J., Dixon, J.F., and Hokin, L.E. (1980)Proc. Natl. Acad. Sci. USA 77, 3292–3296.

    Article  PubMed  CAS  Google Scholar 

  40. Rittenhouse-Simmons, S. (1981)J. Biol. Chem. 256, 4153–4155.

    PubMed  CAS  Google Scholar 

  41. Dudley, D.T., Macfarlane, D.E., and Spector, A.A. (1987)Biochem. J. 246, 669–680.

    PubMed  CAS  Google Scholar 

  42. Weiner, T.W., and Sprecher, H. (1985)J. Biol. Chem. 260, 6032–6038.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Iowa, 4-550 BSB, 52242, Iowa City, IA

    Nagender R. Yerram & Arthur A. Spector

Authors
  1. Nagender R. Yerram
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arthur A. Spector
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Yerram, N.R., Spector, A.A. Effects of omega-3 fatty acids on vascular smooth muscle cells: Reduction in arachidonic acid incorporation into inositol phospholipids. Lipids 24, 594–602 (1989). https://doi.org/10.1007/BF02535075

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02535075

Keywords

Search

Navigation