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Effect of Ca++ on triphosphoinositide extraction in fusing myoblasts

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Lipids

Abstract

Ca++-dependent degradation of triphosphoinositide has been postulated to regulate levels of membrane-bound Ca++ and to generate a 1,2-diacylglycerol fusogen in cell fusion. Triphosphoinositide metabolism was therefore studied during Ca++-induced fusion of cultured chick embryo myoblasts. Using a frequently cited extraction procedure, it was found that apparent Ca++-dependent triphosphoinositide degradation was actually due to inhibition of extraction. A new procedure using the ion-pairing reagent tetrabutylammonium sulfate was developed which was unaffected by Ca++ and gave 2- to 20-fold greater extraction of triphosphoinositide than existing procedures. With this procedure, no changes in triphosphoinositide metabolism were found during myoblast fusion.

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Abbreviations

DMEM:

Dulbecco's modified Eagle's medium

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

TBAS:

tetrabutylammonium sulfate

TPI:

triphosphoinisitide

References

  1. Schacht, J., and Agranoff, B.W. (1972) J. Biol. Chem. 247, 771–777.

    PubMed  CAS  Google Scholar 

  2. Tret'jak, A.G., Limarenko, I.M., Kossova, G.V., Gulak, P.V., and Kozlov, Yu.P. (1977) J. Neurochem. 28, 199–205.

    Article  PubMed  Google Scholar 

  3. Abdel-Latif, A.A., Akhtar, R.A., and Hawthorne, J.N. (1977) Biochem. J. 162, 61–73.

    PubMed  CAS  Google Scholar 

  4. Abdel-Latif, A.A., and Akhtar, R.A. (1978) J. Pharmacol. Exp. Ther. 204, 655–668.

    PubMed  Google Scholar 

  5. Jolles, J., Wirtz, K.W.A., Schotman, P., and Gispen, W.H. (1979) FEBS Lett. 105, 110–114.

    Article  PubMed  CAS  Google Scholar 

  6. Kai, M., and Hawthorne, J.N. (1969) Ann. N.Y. Acad. Sci. 165, 761–773.

    PubMed  CAS  Google Scholar 

  7. Griffin, H.D., Sykes, M., and Hawthorne, J.N. (1979) Biochem. Soc. Trans. 7, 348–353.

    PubMed  CAS  Google Scholar 

  8. Griffin, H.D., and Hawthorne, J.N. (1978) Biochem. J. 176, 541–552.

    PubMed  CAS  Google Scholar 

  9. Allan, D., and Michell, R.H. (1978) Biochim. Biophys. Acta 508, 377–386.

    Google Scholar 

  10. Schimmel, S.D., Kent, C., and Vagelos, P.R. (1977) in Methods in Cell Biology (Prescott, D.M., ed.) Vol. 15, p. 289, Academic Press, New York.

    Google Scholar 

  11. Paterson, B., and Strohman, R.C. (1972) Develop. Biol. 29, 113–138.

    Article  PubMed  CAS  Google Scholar 

  12. Garbus, J., Deluca, H.F., Loomans, M.E., and Strong, F.M. (1963) J. Biol. Chem. 238, 59–63.

    PubMed  CAS  Google Scholar 

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Author notes

  1. Robert I. Grove

    Present address: NIEHS, RM 418 BLD 4, NIH PO Box 12233, 27709, Research Triangle Park, NC

Authors and Affiliations

  1. Departments of Biochemistry and Pharmacology, College of Medicine, University of South Florida, 33612, Tampa, FL

    Robert I. Grove, David Fitzpatrick & Steven D. Schimmel

Authors
  1. Robert I. Grove
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  2. David Fitzpatrick
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  3. Steven D. Schimmel
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Grove, R.I., Fitzpatrick, D. & Schimmel, S.D. Effect of Ca++ on triphosphoinositide extraction in fusing myoblasts. Lipids 16, 691–693 (1981). https://doi.org/10.1007/BF02535065

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  • DOI: https://doi.org/10.1007/BF02535065

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