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Incorporation into lipid classes of products from microsomal desaturation of isomerictrans-octadecenoic acids

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Lipids

An Erratum to this article was published on 01 February 1982

Abstract

The microsomal desaturation of positional isomers oftrans-octadecenoic acids is effected by the Δ9-desaturase and, with concomitant geometric isomerization,cis,trans- andcis,cis-octadecadienoic acids of unusual structure are formed. Incorporation of the substrates and their products into lipids varied from 50.5% for incubations with 14–18∶1 to 81.0% for 6–18∶1. A detailed study of the composition of each of the major lipid classes, i.e., phospholipids, triacylglycerol and cholesteryl esters, as well as the composition of the free fatty acid fraction, revealed a complex picture. Generally, thec,c-18∶2 products were enriched in the phospholipid fraction, whereas thec,t-18∶2 appeared preferentially in cholesteryl esters. The 18∶1 substrates themselves did not show marked preferences for any of the lipid classes. Phospholipase A2 action on phosphatidylcholine and phosphatidylethanolamine demonstrated enrichment of thec,c- and thec,t-18∶2 products in the 2-position, whereas the 18∶1 substrates were preferentially inserted into the 1-positions. Thec,c- andc,t-18∶2 formed by desaturation oft11–18∶1 varied from this pattern, probably due to their conjugated double bond structures. Linoleic acid,c9,c12–18∶2, formed during desaturation oft12–18∶1, surprisingly showed enrichment in the 1-position of phosphatidylcholine. Incubation experiments witht5- andt6-isomers using liver microsomes from rats fed a corn-oil-supplemented diet showed conversion and incorporation rates similar to the rates obtained with microsomes from EFA-deficient rats. The fatty acid composition of lipid classes and the distributions of products and substrate between the 1- and 2-positions of phosphatidylcholine also agreed with results obtained using microsomes from EFA-deficient rats.

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Author notes

  1. T. Riisom

    Present address: Grindsted Products, DK 8220, Brabrand, Denmark

Authors and Affiliations

  1. The Hormel Institute, University of Minnesota, 55912, Austin, MN

    T. Riisom & R. T. Holman

Authors
  1. T. Riisom
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  2. R. T. Holman
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02535188.

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Riisom, T., Holman, R.T. Incorporation into lipid classes of products from microsomal desaturation of isomerictrans-octadecenoic acids. Lipids 16, 647–654 (1981). https://doi.org/10.1007/BF02535059

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  • DOI: https://doi.org/10.1007/BF02535059

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