Abstract
Lipids were examined in whole myelin and 8 myelin subfractions isolated from mouse brain at 18–24, 44–48 and 80–90 days of age. Relative to protein, total lipid was lowest in whole myelin isolated from the oldest animals as well as from subfractions isolated at greater sucrose densities, thus partially accounting for the observed myelin subfraction distribution pattern which shifted during development from an average peak density banding between 0.55 and 0.65 M sucrose to one banding between 0.60 and 0.70 M sucrose. Whole myelin and each myelin subfraction isolated at one age contained nearly the same ratio of sterol and phospholipid to galactolipid; these ratios decreased uniformly during development suggesting enrichment with galactolipid in all myelin subfractions. Sulfatide, as percentage of total galactolipid, was relatively constant during development and appeared to be slightly enriched in the denser myelin subfractions. The findings suggest that regardless of the origin(s) of the subfractions, an age-related mechanism exists in the central nervous system which modifies myelin lipid composition relatively uniformly.
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Matthieu, J.M., Quarles, R.H., Brady, R.O., and Webster, H. DeF. (1973) Biochim. Biophys. Acta 329, 305–317.
Hofteig, J.H., and Druse, M.J. (1976) Life Sci. 18:543–552.
Benjamins, J.A., Miller, K., and Morell, P. (1976a) J. Neurochem. 27, 565–570.
Benjamins, J.A., Gray, M., and Morell, P. (1976b) J. Neurochem. 27, 571–575.
Cuzner, M.L., and Davison, A.N. (1968) Biochem. J. 106, 29–34.
Adams, D.H., and Fox, M.E. (1969) Brain Res. 14, 647–661.
Mehl, E. (1972) Adv. Exp. Biol. Med. 32, 157–170.
McMillan, P.N., Williams, N.I., Kaufman, B., and Day, E.D. (1972) J. Neurochem. 19, 1839–1848.
Benjamins, J.A., Miller, K., and McKhann, G.M. (1973) J. Neurochem. 20, 1589–1603.
Fujimoto, K., Roots, B.I., Burton, R.M., and Agrawal, H.C. (1976) Biochim. Biophys. Acta 426, 659–668.
Sheads, L.D., Eby, M.J., Sampugna, J., and Douglass, L.W. (1977) J. Neurobiol. 8, 67–89.
Bourre, J.M., Pollet, S., Daudu, O., Le Saux, F., and Baumann, N. (1977) Biochimie 59, 819–824.
Waehneldt, T.V. (1978) Brain Res. Bull. 3, 37–44.
Norton, W.T., and Poduslo, S.E. (1973) J. Neurochem. 21, 749–757.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.
Hess, H.H., and Lewin, E. (1965) J. Neurochem. 12, 205–211.
Folch, J., Lees, M., and Sloane-Stanley, G. (1957) J. Biol. Chem. 226, 497–509.
Ames, B. (1966) Methods Enzymol. 8, 115–118.
Rudel, L., and Morris, M. (1973) J. Lipid Res. 14, 364–366.
Rouser, G., Kritchevsky, G., and Yamamoto, A. (1976) in Lipid Chromatographic Analyses (Marinetti, B., ed.) Vol. 3, pp. 713–776, Marcel Dekker Inc., New York, NY.
Kean, E. (1968) J. Lipid Res. 9, 319–327.
Schnedcor, G.W., and Cochran, W.G. (1969) in Statistical Methods, 6th edn., Iowa State University Press, Ames, IA.
Burton, R.M., and Agrawal, H.C. (1975) in Biomembranes-Lipids, Proteins, and Receptors (Burton, R.M., and Packer, L., eds.) pp. 27–50, Proc. Nato Adv. Study Inst., B1-Science Publications Division, Webster Groves, MO.
Reiber, H., and Waehneldt, T.V. (1978) Neurosci. Lett. 8, 177–181.
Linington, C., Waehneldt, T.V., and Neuhoff, V. (1980) Neurosci. Lett. 20, 211–215.
Koul, O., Chou, K.H., and Jungalwala, F.B. (1980) Biochem. J. 186, 959–969.
Agrawal, H.C., Trotter, J.L., Burton, R.M., and Mitchell, R.F. (1974) Biochem. J. 140, 99–109.
Waehneldt, T.V., Mattheiu, J.M., and Neuhoff, V. (1977) Brain Res. 138, 29–43.
Poduslo, S.E. (1975) J. Neurochem. 24,647–654.
Zanetta, J., Zenda, P., Gombos, G., and Morgan, I. (1972) J. Neurochem. 19, 881–883.
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Rhein, L.D., Sampugna, J. Myelin subfractions isolated from mouse brain: Analysis of the lipid composition at three developmental stages. Lipids 16, 502–507 (1981). https://doi.org/10.1007/BF02535048
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DOI: https://doi.org/10.1007/BF02535048