Abstract
Concentrations of albumin in excess of 1% in the incubation mixture inhibited the elongation of added fatty acids and their incorporation into microsomal lipids whereas these reactions were not inhibited with endogenous microsomal membrane fatty acids. The results of these and other studies support the idea that such reactions of membrane lipid fatty acids with membrane-bound enzymes normally occur entirely within the membrane without release of free fatty acids to equilibrate with the fatty acid pool during the process.
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References
Gatt, S., Barenholz, Y., Borkovski-Kubiler, I., and Leibovitz-Ben Gershon, Z. (1972) in Sphingolipids, Sphingolipidases and Allied Disorders (Volk, B.W., and Aronson, S.M., eds.) pp. 237–256, Plenum Press, New York.
Leibovitz-Ben Gershon, Kobiler, I., and Gatt, S. (1972) J. Biol. Chem. 247, 6840–6847.
Barenholz, Y., and Gatt, S. (1972) J. Biol. Chem. 247, 6834–6839.
Gatt, S. (1974) in Enzyme Therapy in Lysosomal Storage Diseases (Tager, J.M., Hooghwinkel, G.J.M., and Daems, W.H., eds.) pp. 207–215, North-Holland Publishing Co., Amsterdam.
Gatt, S., and Bartfai, T. (1975) Israel J. Med. Sci. 11, 1172.
Yedgar, S., and Gatt, S. (1976) Biochemistry 15, 2570–2573.
Gatt, S., and Bartfai, T. (1977) Biochim. Biophys. Acta 488, 1–12.
Gatt, S., and Bartfai, T. (1977) Biochim. Biophys. Acta 488, 13–24.
Dervichian, D.G., and Barque, J.P. (1979) J. Lipid Res. 20, 437–446.
Barque, J.P., and Dervichian, D.G. (1979) J. Lipid Res. 20, 447–455.
Roberts, M.F., Deemer, R.A., Mincey, T.C., and Dennis, E.A. (1977) J. Biol. Chem. 252, 2405–2411.
Gan-Elepano, M., and Mead, J.F. (1978) Biochem. Biophys. Res. Commun. 83, 247–251.
Woelk, H., Rubly, N., Arienti, G., Gani, A., and Porcellati, G. (1981) J. Neurochem. 36, 875–880.
Aeberhard, E.E., Elepano, M.G., and Mead, J.F. (1981) Lipids, in press.
de Goodman, W.S.J. (1958) J. Am. Chem. Soc. 80, 3892–3898.
Yatsu, F.M., and Moss, S. (1971) J. Neurochem. 18, 1895–1901.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.
Folch-Pi, J., Lees, M., and Sloane-Stanley, G.H. (1957) J. Biol. Chem. 226, 497–509.
Dise, C.A., Goodman, D.B.P., and Rasmussen, H. (1980) J. Lipid Res. 21, 292–300.
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Gan-Elepano, M., Aeberhard, E. & Mead, J.F. On the mechanisms of fatty acid transformations in membranes. Lipids 16, 790–795 (1981). https://doi.org/10.1007/BF02535030
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DOI: https://doi.org/10.1007/BF02535030