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Lethal effect ofcis- but nottrans-22-dehydrocholesterol on mouse fibroblast cells

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Lipids

Abstract

cis- andtrans-22-dehydrocholesterol were incorporated into the culture medium for mouse fibroblast cells. Although neither isomer had an effect on sterol biosynthesis, thecis isomer inhibited cell growth and viability and increased Rb+ efflux from the cells. Thetrans isomer had no effect on growth and could replace exogenous cholesterol for growth of cells for which sterol biosynthesis was blocked by 25-hydroxycholesterol.

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Abbreviations

BSA:

bovine serum albumin

SDS:

sodium dodecyl sulfonate

HDMS:

hexamethyldisilazane

TMCS:

trimethylchlorosilane

HMG-CoA:

3-hydroxy-3-methylglutaryl coenzyme A

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Authors and Affiliations

  1. Department of Nutrition and Food Science, College of Agriculture, The University of Arizona, 85721, Tucson, AZ

    Henry W. Kircher & Fumiko U. Rosenstein

  2. The Jackson Laboratory, 04609, Bar Harbor, ME

    Andrew A. Kandutsch

Authors
  1. Henry W. Kircher
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  2. Fumiko U. Rosenstein
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  3. Andrew A. Kandutsch
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Kircher, H.W., Rosenstein, F.U. & Kandutsch, A.A. Lethal effect ofcis- but nottrans-22-dehydrocholesterol on mouse fibroblast cells. Lipids 16, 943–945 (1981). https://doi.org/10.1007/BF02535004

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  • DOI: https://doi.org/10.1007/BF02535004

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