Abstract
Hydroperoxy derivatives of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) can be separated from their respective phospholipids by reverse-phase high performance liquid chromatography (HPLC). However, ultraviolet absorption due to conjugated diene cannot detect the hydroperoxy group. In this work, an electrochemical (EC) detector was first applied to the analysis of hydroperoxy phospholipids. Both the PC and PE hydroperoxides from rat liver were reduced quantitatively by the glassy carbon electrode at −300 mV vs Ag/AgCl. Since neither the hydroxy derivatives nor unoxidized phospholipids showed any response, it would seem this technique can be used to distinguish phospholipid hydroperoxides from their hydroxy derivatives. Thus, the reverse phase HPLC-EC detection method is proposed for the specific analysis of hydroperoxy phospholipids in biological tissues.
Similar content being viewed by others
References
Cathcart, R., Schwiers, E., and Ames, B.N. (1983)Anal. Biochem. 134, 111–116.
Iwaoka, T., and Tabata, F. (1984)FEBS Lett. 178, 47–50.
Yamamoto, Y., Niki, E., Tanimura, R., and Kamiya, Y. (1985)J. Am. Oil Chem. Soc. 62, 1248–1250.
Heath, R. L., and Tappel, A. L. (1976)Anal. Biochem., 76, 184–191.
Yamaguchi, T. (1980)Agric. Biol. Chem. 44, 2747–2748.
Marshall, P. J., Warso, M. A., and Lands, W. E. M. (1985)Anal. Biochem. 145, 192–199.
Porter, N. A., Wolf, R. A., and Weenen, H. (1980)Lipids 15, 163–167.
Crawford, C. G., Platter, R. D., Sessa, D. J., and Rackis, J. J. (1980)Lipids 15, 91–94.
Daolio, S., Traldi, P., Ursini, F., Maiorino, M., and Gregolin, C. (1983)Biomed. Mass Spectrom. 10, 499–504.
Ursini, F., Bonaldo, L., Maiorino, M., and Gregolin, C. (1983)J. Chromatogr. 270, 301–308.
Terao, J., Asano, I., and Matsushita, S. (1984)Arch. Biochem. Biophys. 235, 326–333.
Ursini, F., Maiorino, M., Valente, M., Ferri, L., and Gregolin, C. (1982),Biochim. Biophys. Acta 710, 197–211.
Sohde, K., Izutani, S., and Matsushita, S. (1973)Agric. Biol. Chem. 37, 1979–1981.
Sohde, K., Izutani, S., and Matsushita, S. (1973)Nippon Nogeikagaku Kaishi 47, 587–589.
Kissinger, P. T., Refshauge, C., Dreiling, R., and Adams, R. N. (1973)Anal. Lett. 6, 465–477.
Terao, J., Asano, I., and Matsushita, S. (1985)Lipids 20, 312–317.
Sevanian, A., Mulkkassah-Kelly, S. F., and Montestruque, S. (1983)Arch. Biochem. Biophys. 223, 441–452.
Grossmann, A., and Wendel, A. (1983)Eur. J. Biochem. 135, 549–552.
Author information
Authors and Affiliations
About this article
Cite this article
Yamada, K., Terao, J. & Matsushita, S. Electrochemical detection of phospholipid hydroperoxides in reverse-phase high performance liquid chromatography. Lipids 22, 125–128 (1987). https://doi.org/10.1007/BF02534865
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534865