Abstract
Capillary gas liquid chromatography analyses were conducted on free and esterified sterol fractions of cotton (Gossypium hirsutum cv. Stoneville 213) floral buds and anthers. The free sterols of both cotton buds and anthers consist mainly of the common plant sterols sitosterol, stigmasterol and 24ζ-methylcholest-5-en-3β-ol. The composition of esterified sterols of cotton buds and anthers were similar, and consisted of pollinastanol, 31-norcycloartanol, cycloartenol, 31-norcycloartenol, 24-dehydropolinastanol and sitosterol. Desmosterol was also present in both the free and esterified sterols of anthers. The identities of the sterols were confirmed by gas chromatography-mass spectrometry analyses. Esterified sterols accounted for 46.7 and 88.7% of total sterols of cotton bud and anthers, respectively. The ratio of esterified sterol to free sterol per gram of tissue is about 8∶1 in anthers. The Δ5-sterols of the esterified sterols of cotton buds and anthers account for only 17 and 9.2% of the total sterols, respectively.
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Abbreviations
- 24α-ethylcholest-5-en-3β-ol:
-
24ζ-methylcholest-5-en-3β-ol
- stigmasterol:
-
24α-ethylcholesta-5,22E-dien-3β-ol
- desmosterol:
-
cholesta-5,24-dien-3β-ol
- pollinastanol:
-
9β,19-cyclo-14α-methyl-5α-cholestan-3β-ol
- 24-dehydropollinastanol:
-
9β,19-cyclo-14α-methyl-5α-cholest-24-en-3β-ol
- cycloartenol:
-
9β,19-cyclo-4,4-14α-trimethyl-5α-cholest-24-en-3β-ol
- 31-norcycloartanol:
-
9β,19-cyclo-4α-14α-dimethyl-5α-cholestan-3β-ol
- 31-norcycloartenol:
-
9β,19-cyclo-4α,14α-dimethyl-5α-cholest-24-en-3β-ol
- 24-methylenecholesterol:
-
24-methylchoesta-5,24(28)-dien-3β-ol
References
McKibben, G.H., Thompson, M.J., Parrott, W.L., Thompson, A.C., and Lusby, W.R. (1985)J. Chem. Ecol. 11, 1229–1238.
Thompson, A.C., Henson, R.D., Gueldner, R.C., and Hedin, P.A. (1970)Lipids 5, 283–284.
Svoboda, J.A., and Thompson, M.J. (1985) incomprehensive Insect Physiology Biochemistry and Pharmacology (Kerkut, G.A., and Gilbert, L.I., eds.) Vol. 10, pp. 137–175, Pergamon Press, Oxford.
Earle, N.W., Lambremont, E.N., Burks, M.L., Slatten, B.H., and Bennett, A.F. (1967)J. Econ. Entomol. 60, 291–293.
Sliwowski, J.K., and Caspi, E. (1977)J. Steroid Biochem. 8, 42–49.
Nes, W.R., and McKean, M.L. (1977)Biochemistry of Steroids and Other Isopentenoids pp. 241, 482, 538, University Park Press, Baltimore, MD.
Patterson, G.W. (1971)Anal. Chem. 43, 1165–1170.
Thompson, M.J., Patterson, G.W., Dutky, S.R., Svoboda, J.A., and Kaplanis, J.N. (1980)Lipids 15, 719–733.
Doyle, P.J., Patterson, G.W., Dutky, S.R., and Cohen, C.F. (1971)Phytochemistry 10, 2093–2098.
Thompson, M.J., Dutky, S.R., Lehner, Y., Standifier, L.N., and Herbert, E.W. Jr. (1978)Phytochemistry 17, 1053–1056.
Hugel, M.-F., Barbier, M., and Lederer, E. (1964)Bull. Soc. Chim. Fr., 2012–2013.
Devys, M., Andre, D., and Barbier, M. (1969)Compt. Rend. Acad. Sci. D, 269, 798–801.
Barbier, M., Hugel, M.-F., and Lederer, E. (1960)Bull. Soc. Chim. Biol. 42, 91–97.
Hugel, M.-F., Vetter, W., Audier, H., Barbier, M., and Lederer, E. (1964)Phytochemistry 3, 7–16.
Devys, M., and Barbier, M. (1965)Compt. Rend. Acad. Sci. D, 261, 4901–4903.
Heupel, R.C., Sauvaire, Y., Le, P.H., Parish, E.L., and Nes, W.D. (1986)Lipids 21, 69–75.
Fenner, G.P., Patterson, G.W., and Koines, P.M. (1986)Lipids 21, 49–51.
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Lusby, W.R., Oliver, J.E., McKibben, G.H. et al. Free and esterified sterols of cotton buds and anthers. Lipids 22, 80–83 (1987). https://doi.org/10.1007/BF02534857
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DOI: https://doi.org/10.1007/BF02534857