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A modified radiometric assay for 3-hydroxy-3-methylglutaryl coenzyme a reductase

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Lipids

Abstract

A radiometric assay for measuring the activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is described. The assay is based on the separation of the mevalonate product from HMG-CoA by high-voltage electrophoresis. This method is more sensitive and more specific than the NADPH-based spectrophotometric assay, and less tedious than available radiometric assays. It has been used to measure HMG-CoA reductase activity in crude extracts ofSaccharomyces cerevisiae and in human skin fibroblasts.

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Author notes

  1. Nafsika H. Georgopapadakou & Barbara A. Dix

    Present address: Hoffman-La Roche, Inc., 07110, Nutley, NJ

Authors and Affiliations

  1. The Squibb Institute for Medical Research, 08540, Princeton, New Jersey

    Nafsika H. Georgopapadakou & Barbara A. Dix

Authors
  1. Nafsika H. Georgopapadakou
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  2. Barbara A. Dix
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Georgopapadakou, N.H., Dix, B.A. A modified radiometric assay for 3-hydroxy-3-methylglutaryl coenzyme a reductase. Lipids 19, 966–970 (1984). https://doi.org/10.1007/BF02534735

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  • DOI: https://doi.org/10.1007/BF02534735

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