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Lipid and fatty acid characterization and metabolism in the sea anemonePhymactis clematis (Dana)

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Lipids

Abstract

Neutral lipid, phospholipids and fatty acids of the sea anemonePhymactis clematis from the south-west Atlantic were characterized and quantified in spring and autumn. Neutral lipids predominated over phospholipids in both seasons. Triacylglycerol and diacylglycerol ethers were the major lipids. In spring, an increase of esterified sterols was noted. The major fatty acids found were 22∶5ω3, 20∶5ω3 and 16∶0. The sea anemones were also incubated in vivo with either [1-14C]linoleate or [1-14C] α-linolenate for 2 hr. Isotope incorporation into lipids and their transformations into higher fatty acids were examined. Both precursors were incorporated into the lipids, mainly in triacylglycerols and mono-acylglycerols, while α-linolenate was also incorporated into phospholipids. The radioactive linoleate was elongated to 20∶2, 22∶2 and 24∶2 fatty acids, but not desaturated to 18∶3ω6. α-Linolenate was desaturated by Δ6 desaturase to 18∶4ω3. The specificity of Δ6-desaturase is discussed.

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Author notes

  1. Ricardo J. Pollero (Member)

    Present address: Carrera del Investigador Científico, Comisión de Investigaciones Científicas de la Prov. de Buenos Aires, Argentina

Authors and Affiliations

  1. Facultad de Ciencias Médicas, Instituto de Investigaciones Bioquimicas de la Plata, CONICET-UNLP, Calles 60 y 120, 1900, La Plata, Argentina

    Ricardo J. Pollero (Member)

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  1. Ricardo J. Pollero
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Pollero, R.J. Lipid and fatty acid characterization and metabolism in the sea anemonePhymactis clematis (Dana). Lipids 18, 12–17 (1983). https://doi.org/10.1007/BF02534684

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  • DOI: https://doi.org/10.1007/BF02534684

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