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Effect of methylmercuric chloride on gangliosides of mouse neuroblastoma cells in culture

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Lipids

Abstract

The effect of methylmercuric chloride (CH3 HgCl) on the levels of gangliosides in mouse neuroblastoma cells (NBP2) in culture was studied. The treatment of NB cells with low concentrations (0.1 μM and 0.2 μM) of CH3 HgCl, which did not affect the growth rate or morphology, caused an increase in the level of the GM3 ganglioside without changing the level of other gangliosides. The treatment of NB cells with higher concentrations (0.5 μM and 1 μM) of CH3 HgCl, which inhibited the growth of NB cells, caused a decrease in the level of GM3 and an increase in the level of GM2. These results show that alterations in the levels of specific gangliosides can be observed in cells which do not exhibit any detectable change in growth rate or morphology. This change may be associated with subtle changes in brain functions, including behavioral and psychological changes, after exposure to low concentrations of organic mercury.

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Authors and Affiliations

  1. Centre de Neurochimie, Centre National de la Recherche Scientifigue, Strasbourg, France

    G. Rebel

  2. Center for Vitamins and Cancer Research, Department of Radiology, School of Medicine, University of Colorado Health Sciences Center, 80262, Denver, CO

    P. Guerin & K. N. Prasad

Authors
  1. G. Rebel
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  2. P. Guerin
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  3. K. N. Prasad
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Rebel, G., Guerin, P. & Prasad, K.N. Effect of methylmercuric chloride on gangliosides of mouse neuroblastoma cells in culture. Lipids 18, 664–667 (1983). https://doi.org/10.1007/BF02534680

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  • DOI: https://doi.org/10.1007/BF02534680

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