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Use of antioxidants in the analysis of vatamins A and E in mammalian plasma by high performance liquid chromatography

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Lipids

Abstract

A simple, sensitive, quantitative method for the simultaneous assay of retinol, α-tocopherol and γ-tocopherol in rat, guinea pig, monkey and human plasma was developed by using high performance liquid chromatography. It was found that antioxidant was required to stabilize the fat-soluble vitamins in the plasma of rats. The effect of several antioxidants on the recovery of fat-soluble vitamins was evaluated. Results showed that 0.125% butylated hydroxytoluene (BHT) in ethanol and 0.025% BHT in heptane yielded recoveries >95% in 0.1 ml plasma.

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Authors and Affiliations

  1. Nutrients Research Unit, US Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 94710, Berkeley, CA

    Faye I. Chow & Stanley T. Omeye

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  1. Faye I. Chow
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  2. Stanley T. Omeye
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Chow, F.I., Omeye, S.T. Use of antioxidants in the analysis of vatamins A and E in mammalian plasma by high performance liquid chromatography. Lipids 18, 837–841 (1983). https://doi.org/10.1007/BF02534644

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  • DOI: https://doi.org/10.1007/BF02534644

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