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1-O-alkyl-linked phosphoglycerides of human platelets: Distribution of arachidonate and other acyl residues in the ether-linked and diacyl species

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Lipids

An Erratum to this article was published on 01 January 1984

Abstract

In this study, the 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine content of human platelets was determined. The distribution of arachidonate among the 1,2-diacyl, 1-O-alkyl-2-acyl, and 1-O-alk-l′-enyl-2-acyl classes of choline- and ethanolamine-containing phosphoglycerides was also assessed. The major platelet phospholipids were choline-containing phosphoglycerides (38%), ethanolamine-containing phosphoglycerides (25%) and sphingomyelin (18%), with smaller amounts of phosphatidylserine (11%) and phosphatidylinositol (4%). In addition to the diacyl class, the choline-linked fraction was found to contain both 1-O-alkyl-2-acyl (10%) and 1-O-alk-l′-enyl-2-acyl (9%) species. The ethanolamine-linked fraction, on the other hand, had an elevated level of the 1-O-alk-l′-enyl-2-acyl (60%) species and a small amount of the 1-O-alkyl-2-acyl component (4%). The major fatty acyl residues found in all classes of the choline and ethanolamine phospholipids were 16∶0, 18∶0, (Δ9), 18∶2(n−6) and 20∶4(n−6). The 1-O-alk-l and 1-O-alk-l′-enyl fraction of the ethanolamine-linked phospholipids also contained substantial amounts of 22∶4(n−6), 22∶5(n−3) and 22∶6(n−3) acyl chains. Arachidonate comprised 44% of the acyl residues in thesn-2 position of 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine. Corresponding values for the diacyl and 1-O-alk-l′-enyl-2-acyl species were 23% and 25%, respectively, based on all 20∶4(n−6) being linked to thesn-2 position of all classes. In the ethanolamine-linked phosphoglycerides, arachidonate constituted 60%, 20% and 68% of the acyl groups in thesn-2 position of the 1,2-diacyl, 1-O-alkyl-2-acyl and 1-O-alk-l′-enyl-2-acyl classes, respectively. The content of 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine appears sufficient to support the synthesis of platelet activating factor by a deacylation-reacylation pathway in platelets. Our findings also demonstrate that human platelets contain a significant amount of 1-O-alkyl-2-arachidonyl-sn-glycero-3-phosphocholine that could possibly serve as a precursor of both platelet activating factor and bioactive arachidonate metabolites.

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Abbreviations

PAF:

platelet activating factor

GPC:

sn-glycero-3-phosphocholine

TLC:

thin layer chromatography

PRP:

platelet-rich plasma

PC:

choline-linked phosphoglycerides

PE:

ethanolamine-linked phosphoglycerides

GPE:

sn-glycero-3-phosphoethanolamine

GLC:

gas liquid chromatography

HPLC:

high performance liquid chromatography

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Authors and Affiliations

  1. Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, 27103, Winston-Salem, NC

    Howard W. Mueller & Robert L. Wykle

  2. Specialized Center for Thrombosis Research, Temple University Medical School, 19140, Philadelphia, PA

    A. David Purdon & J. Bryan Smith

Authors
  1. Howard W. Mueller
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  2. A. David Purdon
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  3. J. Bryan Smith
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  4. Robert L. Wykle
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02534613.

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Mueller, H.W., Purdon, A.D., Smith, J.B. et al. 1-O-alkyl-linked phosphoglycerides of human platelets: Distribution of arachidonate and other acyl residues in the ether-linked and diacyl species. Lipids 18, 814–819 (1983). https://doi.org/10.1007/BF02534641

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  • DOI: https://doi.org/10.1007/BF02534641

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