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Effect of dietary fatty acids on Δ5 desaturase activity and biosynthesis of arachidonic acid in rat liver microsomes

  • Published:
Lipids

Abstract

The effect of different fatty acids supplemented to a fat-free diet on the activity of Δ5 desaturase was studied. Fat-free diet produces a reduction in the conversion of eicosa-8,11,14-trienoic acid to arachidonic acid. The addition of thecis-ω6 acids, linoleic, γ-linolenic or arachidonic to the diet produces an increase of eicosatrienoic acid desaturation, shifting Δ5 desaturase activity towards the controls on a balanced diet. This reactivation is apparently produced by induction of enzyme biosynthesis since linoleate effect was suppressed by simultaneous cycloheximide injection. On the contrary, no changes in Δ5 desaturation activity were found when the diet was supplemented with palmitic or 9-trans,12-trans-linoleic acid. The changes on the activity of Δ5 desaturase were compared with the fatty acid composition of plasma and liver microsomes.

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Author notes

  1. I. N. T. De Gómez Dumm (Members), M. J. T. De Alaniz & R. R. Brenner

    Present address: Carrera del Investigador Científico, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina

Authors and Affiliations

  1. Instituto de Investigaciones Bioquimicas de La Plata (INIBIOLP), CONICET-UNLP, Facultad de Ciencias Médicas, Calles 60 y 120, 1900, La Plata, Argentina

    I. N. T. De Gómez Dumm (Members), M. J. T. De Alaniz & R. R. Brenner

Authors
  1. I. N. T. De Gómez Dumm
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  2. M. J. T. De Alaniz
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  3. R. R. Brenner
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De Gómez Dumm, I.N.T., De Alaniz, M.J.T. & Brenner, R.R. Effect of dietary fatty acids on Δ5 desaturase activity and biosynthesis of arachidonic acid in rat liver microsomes. Lipids 18, 781–788 (1983). https://doi.org/10.1007/BF02534636

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  • DOI: https://doi.org/10.1007/BF02534636

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