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Quantitative determination of Tri-, Di-,monooleins and free oleic acid by the thin layer chromatography-flame lonization detector system using internal standards and boric acid impregnated chromarod

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Lipids

Abstract

The separation conditions for hydrolysates of triglycerides by lipase and their quantitative determination are discussed for a thin layer chromatography-flame ionization detector system utilizing internal standards. The complete separation of glyceride hydrolysis mixtures (triolein 1,3-diolein, 1,2-diolein, 1-monoolein and oleic acid) was achieved on a 3% boric acid-impregnated Chromarod S-II by development with benzene/chloroform/acetic acid (70∶30∶2, v/v/v) (mobile phase A) or hexane/ ether/acetic acid (70∶30∶1, v/v/v) (mobile phase B). Mobile phase B had an advantage over mobile phase A in terms of free space to add internal standards for simultaneous quantitation and was employed.p-Hydroxybenzoic acid andp-carboethoxy benzyl alcohol, which appeared between 1,2-diolein and 1-moloolein, were adopted as the internal standards. The calibration curves relating internal standards to each glyceride were all approximated by the equations Y=aXb giving high correlations. The method was applied to hydrolysis of triolein by pancreatic lipase.

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Abbreviations

TO:

Triolein

DO:

diolein

MO:

monoolein

OA:

oleic acid

HB:

p-hydroxybenzoic acid

CEB:

p-carboethoxy benzyl alcohol

PVA:

polyvinyl alcohol

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Authors and Affiliations

  1. Department of Clothing and Textiles, Faculty of the Science of Living, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, 558, Osaka, Japan

    Takako Tatara, Tomiko Fujii, Tokuzo Kawase & Motoi Minagawa

Authors
  1. Takako Tatara
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  2. Tomiko Fujii
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  3. Tokuzo Kawase
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  4. Motoi Minagawa
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Additional information

Part of this investigation was reported at the annual meeting of JOCS in Tokyo, November 1982.

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Tatara, T., Fujii, T., Kawase, T. et al. Quantitative determination of Tri-, Di-,monooleins and free oleic acid by the thin layer chromatography-flame lonization detector system using internal standards and boric acid impregnated chromarod. Lipids 18, 732–736 (1983). https://doi.org/10.1007/BF02534541

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  • DOI: https://doi.org/10.1007/BF02534541

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