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(n−3) and (n−6) polyunsaturated fatty acids in the phosphoglycerides of salt-secreting epithelia from two marine fish species

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Lipids

Abstract

Fatty acid analyses were carried out on phosphoglycerides isolated from microsomal fractions of the rectal gland of the dogfish,Scyliorthinus canicula, and gills of the cod,Gadus morhua. Ratios of (n−3)/(n−6) polyunsaturated fatty acids were ca. 10 for phosphatidylcholine, (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) from cod gills, reflecting high concentrations of 20∶5 (n−3) and 22∶6(n−3). The ratio for phosphatidylinositol (PI) from cod gills was 1.3, reflecting high concentrations of 20∶4(n−6) as well as (n−3) polyunsaturates. PC, PE and PS from rectal glands all had much lower (n−3)/(n−6) ratios than in cod gills, reflecting higher concentrations of 20∶4(n−6), but the lowest ratio was again present in PI. The latter phospholipid had high concentrations of 18∶0 in both tissues. The relative constancy of the fatty acid composition of PI in the two salt-secreting tissues and its similarity to mammalian phospholipids is considered to reflect its specialized role in biomembranes.

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References

  1. Keys, A., and Willmer, E.N. (1932) J. Physiol. 76, 368–378.

    PubMed  CAS  Google Scholar 

  2. Hokin, L.E., Dahl, J.L., Deupree, J.D., Dixon, J.F., Hackney, J.F., and Perdue, J.F. (1973) J. Biol. Chem. 248, 2593–2605.

    PubMed  CAS  Google Scholar 

  3. Hokin, L.E., and Hokin, M.R. (1959) Nature 184, 1068–1069.

    Article  PubMed  CAS  Google Scholar 

  4. Payan, P., Matty, A.J., and Maetz, J. (1975) J. Comp. Physiol. 104, 33–48.

    CAS  Google Scholar 

  5. Pic, P., Mayer-Gostan, N., and Maetz, J. (1975) Am. J. Physiol. 228, 441–447.

    PubMed  CAS  Google Scholar 

  6. Girard, J.P., Thomson, A.J., and Sargent, J.R. (1977) Febs. Lett. 73, 267–270.

    Article  PubMed  CAS  Google Scholar 

  7. Stoff, J.S., Silva, P., Field, M., Forrest, J.M., Stevens, A., and Epstein, F.H. (1977) J. Exp. Zool. 199, 443–448.

    Article  PubMed  CAS  Google Scholar 

  8. Djabali, M., Payan, P., Girard, J.P., and Pic, P. (1981) C.R. Acad. Sci. Paris 293, 333–336.

    CAS  Google Scholar 

  9. Marshall, P.J., Boatman, D.E., and Hokin, L.E. (1981) J. Biol. Chem. 256, 844–847.

    PubMed  CAS  Google Scholar 

  10. Irvine, R.F. (1982) Biochem. J. 204, 3–16.

    PubMed  CAS  Google Scholar 

  11. Rubin, R.P., Sink, L.E., and Freer, R.J. (1981) Biochem. J. 194, 497–505.

    PubMed  CAS  Google Scholar 

  12. Pic, P. (1975) J. Physiol. (Paris) 71, 146A.

    Google Scholar 

  13. Anderson, A.A., Fletcher, T.C., and Smith, G.M. (1981) Comp. Biochem. Physiol. 70 C, 195–199.

    Article  CAS  Google Scholar 

  14. Ackman, R.G. (1980) in Advances in Fish Science and Technology (Connell, J.J., ed.) pp. 86–103, Fishing News Books Ltd., Farnham, Surrey, UK.

    Google Scholar 

  15. Cowey, C.B., and Sargent, J.R. (1972) in Advances in Marine Biology, (Russell, F.S., and Yonge, M., eds.) Vol. 10, pp. 419–448, Academic Press, London.

    Google Scholar 

  16. Holman, R.T. (1968) in Progress in the Chemistry of Fats and Other Lipids, (Holman, R.T., ed.) Vol. IX, pp. 275–348, Pergamon Press, London.

    Google Scholar 

  17. Burr, G.O., and Burr, M.M. (1929) J. Biol. Chem. 82, 345–367.

    CAS  Google Scholar 

  18. Burr, G.O., and Burr, M.M. (1930) J. Biol. Chem. 86, 587–621.

    CAS  Google Scholar 

  19. Parsons, J.G., and Patton, S. (1967) J. Lipid Res. 8, 696–698.

    PubMed  CAS  Google Scholar 

  20. Christie, W.W. (1973) Lipid Analysis, Pergamon Press, Oxford.

    Google Scholar 

  21. Ackman, R.G., and Eaton, C.A. (1978) Fett Seifen Anstrichm. 80, 21–37.

    Article  CAS  Google Scholar 

  22. Philpott, C.W., and Copeland, D.E. (1963) J. Cell Biol. 18, 389–404.

    Article  PubMed  CAS  Google Scholar 

  23. Ritch, R., and Philpott, C.W. (1969) Exp. Cell Res. 55, 17–24.

    Article  PubMed  CAS  Google Scholar 

  24. Bell, M.V., and Sargent, J.R. (1979) Biochem. J. 179, 431–438.

    PubMed  CAS  Google Scholar 

  25. Addison, R.F., and Ackman, R.G. (1971) Can. J. Biochem. 49, 873–876.

    Article  PubMed  CAS  Google Scholar 

  26. Chacko, G.K., Goldman, D.E., and Pennock, B.E. (1972) Biochim. Biophys. Acta 280, 1–16.

    PubMed  CAS  Google Scholar 

  27. Bergh, C.H., Larson, G., and Samuelsson, B.E. (1975) Lipids 10, 299–302.

    Article  PubMed  CAS  Google Scholar 

  28. Sargent, J.R., and Whitte, K.J. (1981) in Analysis of Marine Ecosystems (Longhurst, A.R., ed.) pp. 491–533 Academic Press, London.

    Google Scholar 

  29. Takagi, T., Easton, C.A., and Ackman, R.G. (1980) Can. J. Fish. Aquat. Sci. 37, 195–202.

    Article  CAS  Google Scholar 

  30. Sargent, J.R., Falk-Petersen, I.B., and Calder, A.G. (1983) Mar. Biol. 72, 257–264.

    Article  CAS  Google Scholar 

  31. Hazel, J.R. (1979) Am. J. Physiol. 236, R91-R101.

    PubMed  CAS  Google Scholar 

  32. Castledine, A.J., and Buckley, J.T. (1982) Comp. Biochem. Physiol. 71B, 119–126.

    CAS  Google Scholar 

  33. Yu, T.C., Sinnhuber, R.O., and Hendricks, J.D. (1979) Lipids 14, 572–575.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Natural Environment Research Council, Institute of Marine Biochemistry, St. Fittick's Road, AB1 3RA, Aberdeen, UK

    Michael V. Bell, Catriona M. F. Simpson & John R. Sargent

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  1. Michael V. Bell
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  2. Catriona M. F. Simpson
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  3. John R. Sargent
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Bell, M.V., Simpson, C.M.F. & Sargent, J.R. (n−3) and (n−6) polyunsaturated fatty acids in the phosphoglycerides of salt-secreting epithelia from two marine fish species. Lipids 18, 720–726 (1983). https://doi.org/10.1007/BF02534539

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  • DOI: https://doi.org/10.1007/BF02534539

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