Skip to main content
SpringerLink
Log in

Urinary prostaglandin E2 and vasopressin excretion in essential fatty acid-deficient rats: Effect of linolenic acid supplementation

  • Published:
Lipids

Abstract

Three groups of weanling male rats were fed on a fat-free diet for 13 weeks. One group received only the fat-free diet (FF rats), the other 2 groups received the fat-free diet and a daily supplement of 2 energy% ethyl linoleate ([n−6] rats), or 2 energy% ethyl linolenate ([n−3] rats). Urinary excretion of prostaglandin E2 (PGE2), immunoreactive arginine vasopressin (iA VP), and kallikrein were determined. PGE2 was quantitated with a radioimmunoassay having 4.9% cross-reactivity with prostaglandin E3 (PGE3). After 4 weeks on the diet, water consumption and urinary iAVP excretion increased significantly in the FF rats and the (n−3) rats compared with the (n−6) rats. Urinary PGE2 excretion was the same for all 3 groups during the first 10 weeks; thereafter it decreased in FF rats and (n−3) rats compared with the (n−6) rats. There was no difference in urinary PGE2 excretion between the FF rats and the (n−3) rats, even though large differences were found in the percentage of arachidonic acid (20∶4[n−6]), icosapentaenoic acid (20∶5[n−3]), and icosatrienoic acid (20∶3[n−9]) of total kidney fatty acids as well as of kidney phosphatidylinositol fatty acids. Fractionation of urine extracts on high performance liquid chromatography with radioimmunoassay detection indicated that (n−3) rats excreted very little PGE3, if any. Urine output followed the same pattern, as did urinary PGE2 excretion. Urinary kallikrein was estimated at week 12 only. It was found to be significantly lower in FF rats and (n−3) rats. Increased water consumption and increased urinary iAVP excretion seem to be early symptoms (after 4 weeks) of EFA deficiency, whereas decreased urine output and decreased urinary PGE2 excretion occur much later (after 10 weeks). Two energy% linolenate supplementation to a fat-free diet did not change the appearance of any of the measured EFA-deficiency symptoms except for a slightly improved growth rate. There was no evidence of a significant urinary PGE3 excretion in spite of an extreme enrichment of kidney lipids with 20∶5(n−3). It is suggested that urinary PGE2 is derived from precursors delivered from an arachidonic acid pool, which is rather resistant to restriction in dietary linoleate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EFA:

essential fatty acid

PGE2, PGE3 :

prostaglandin E2, prostaglandin E3, etc

iAVP:

immunoreactive arginine-vasopressin

HPLC:

high performance liquid chromatography

TLC:

thin layer chromatography

PI:

phosphatidylinositol

References

  1. Hansen, H.S. (1981) Lipids 16, 849–854.

    Article  PubMed  CAS  Google Scholar 

  2. Hansen, H.S. (1982) Lipids 17, 321–322.

    PubMed  CAS  Google Scholar 

  3. Beck, T.R., and Dunn, M.J. (1981) Miner. Electrolyte Metab. 6, 46–59.

    CAS  Google Scholar 

  4. Stokes, J.B. (1981) Am. J. Physiol. 240, F471-F480.

    PubMed  CAS  Google Scholar 

  5. Dunn, M.J., and Hood, V.L. (1977) Am. J. Physiol. 233, F169-F184.

    CAS  Google Scholar 

  6. Aaes-Jørgensen, E. (1961) Physiol. Rev. 41, 1–51.

    PubMed  Google Scholar 

  7. Lundberg, W.O. (1979) Fette Seifen Anstrichm. 81, 337–348.

    Article  CAS  Google Scholar 

  8. Struijk, C.B., Beerthuis, R.K., Pabon, H.J.J., and van Dorp, D.A. (1966) Recl. Trav. Chim. 85, 1233–1250.

    CAS  Google Scholar 

  9. Smith, D.R., Weatherly, B.C., Salmon, J.A., Ubatuba, F.B., Gryglewski, R.J., and Moncada, S. (1979) Prostaglandins 18, 423–438.

    Article  PubMed  CAS  Google Scholar 

  10. Lands, W.E.M., LeTellier, P.R., Rome, L.H., and Vanderhoek, J.V. (1973) Adv. Biosci. 9, 15–28.

    Google Scholar 

  11. ten Hoor, F., de Deckere, E.A.M., Haddeman, E., Hornstra, G., and Quadt, J.F.A. (1980) Adv. Prostagl. Thrombox. Res. 8, 1771–1781.

    Google Scholar 

  12. Hansen, H.S. (1983) World Review Nutr. Dietetics (in press).

  13. Aaes-Jørgensen, E., and Hølmer, G. (1969) Lipids 4, 501–506.

    Article  PubMed  Google Scholar 

  14. Jensen, B. (1976) Lipids 11, 179–188.

    Article  PubMed  CAS  Google Scholar 

  15. Laboratory Animal Handbook 2 (1969), Laboratory Animals Ltd., London.

  16. Hansen, H.S., Fjalland, B., and Jensen, B. (1983) Lipids 18, xxx-xxx.

    Google Scholar 

  17. Christensen, P., and Leyssac, P.P. (1976) Prostaglandins 11, 399–420.

    Article  PubMed  CAS  Google Scholar 

  18. Christensen, J.D., and Jensen, S.E. (1978) Acta Endocrinol. 87, 283–291.

    PubMed  CAS  Google Scholar 

  19. Bygdeman, M., and Samuelsson, B. (1966) Clin. Chim. Acta 13, 465–474.

    Article  PubMed  CAS  Google Scholar 

  20. Powell, W.S. (1982) Methods Enzymol. 86, 467–477.

    PubMed  CAS  Google Scholar 

  21. Olsen, U.B. (1978) Acta Physiol. Scand. 104, 443–452.

    Article  PubMed  CAS  Google Scholar 

  22. Hedegaard, E., and Jensen, B. (1981) J. Chromatogr. 225, 450–454.

    PubMed  CAS  Google Scholar 

  23. Galli, C., Agradi, E., Petroni, A., and Tremoli, E. (1981) in Prostaglandins and Cardiovascular Disease (Hegyeli, R.J., ed.) pp. 87–99, Raven Press, New York.

    Google Scholar 

  24. Galli, C., and Racagni, G. (1982) Methods Enzymol. 86, 635–642.

    Article  PubMed  CAS  Google Scholar 

  25. Bell, R.L., Kennerly, D.A., Stanford, N., and Majerus, P.W. (1979) Proc. Natl. Acad. Sci. USA 76, 3238–3241.

    Article  PubMed  CAS  Google Scholar 

  26. Gréen, K. (1971) Biochemistry 10, 1072–1086.

    Article  PubMed  Google Scholar 

  27. Ferretti, A., Schoene, N.W., and Flanagan, V.P. (1981) Lipids 16, 866–869.

    Article  PubMed  Google Scholar 

  28. Schoene, N.W., Ferretti, A., and Fiore, D. (1981) Lipids 16, 866–869.

    Article  PubMed  CAS  Google Scholar 

  29. Al-Nagdy, S.A., Al-Gauhari, A.A.M., El-Sabbagh, M.E., and Eisa, E.A. (1974) Comp. Biochem. Physiol. 47A, 835–843.

    Article  Google Scholar 

  30. Sakr, H.M., and Dunham, E.W. (1982) Am. J. Physiol. 243, H61-H67.

    PubMed  CAS  Google Scholar 

  31. Nasjletti, A., and Malik, K.U. (1981) Kidney Int. 19, 860–868.

    PubMed  CAS  Google Scholar 

  32. Mohrhauer, H., and Holman, R.T. (1963) J. Lipid Res. 4, 151–159.

    PubMed  CAS  Google Scholar 

  33. Thomasson, H.J. (1953) Int. Rev. Vitam. Res. 25, 62–82.

    CAS  Google Scholar 

  34. Houtsmüller, U.M.T., and van der Beek, A. (1981) Progr. Lipid Res. 20, 219–224.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemical Laboratory, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100, Copenhagen, Denmark

    Harald S. Hansen & Benny Jensen

Authors
  1. Harald S. Hansen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Benny Jensen
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Hansen, H.S., Jensen, B. Urinary prostaglandin E2 and vasopressin excretion in essential fatty acid-deficient rats: Effect of linolenic acid supplementation. Lipids 18, 682–690 (1983). https://doi.org/10.1007/BF02534534

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02534534

Keywords

Search

Navigation