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Studies of triacyglycerol structure of very low density lipoproteins of normolipemic subjects and patients with type III and type IV hyperlipoproteinemia

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Lipids

Abstract

The triacylglycerols of very low density lipoproteins (VLDL-TG) were analyzed in samples from normal subjects and patients with Frederickson’s Type III and Type IV hyperlipoproteinemia. VLDL were obtained by conventional ultracentrifugation, and the triacylglycerols were isolated by thin-layer chromatography (TLC). Representative sn-1,2(2,3)- and sn-1,3-diacylglycerols were generated by Grignard degradation of the triacylglycerols, and were resolved by TLC on borate-treated silica gel. The molecular association of the fatty acids in the diacylglycerol moieties was determined by gasliquid chromatography with mass spectrometry (GC/MS) of the tertiary-butyldimethylsilyl ethers. The positional distribution of the fatty acids was established by the Brockerhoff stereospecific analysis. The results showed a marked asymmetry in the distribution of the fatty acids in all samples, with the saturated acids predominantly in the sn-1-position and the unsaturated fatty acids distributed about equally between the sn-2- and sn-3-positions. In all instances, the molecular species composition of the sn-1,2-, sn-2,3- and sn-1,3-diacylglycerols was found to be similar to that calculated for 1-random 2-random 3-random distribution of triacylglycerols. There were marked differences in the quantitative composition of the molecular species of the VLDL-TG between normal subjects and patients, but these discrepancies were attributed to differences in the fatty acid composition of the samples.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research, University of Toronto, M5G 1L6, Toronto, Canada

    J. J. Myher, A. Kuksis, W. C. Breckenridge & J. A. Little

  2. Department of Medicine, University of Toronto, M5G 1L6, Toronto, Canada

    J. J. Myher, A. Kuksis, W. C. Breckenridge & J. A. Little

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  1. J. J. Myher
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  2. A. Kuksis
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  3. W. C. Breckenridge
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  4. J. A. Little
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Myher, J.J., Kuksis, A., Breckenridge, W.C. et al. Studies of triacyglycerol structure of very low density lipoproteins of normolipemic subjects and patients with type III and type IV hyperlipoproteinemia. Lipids 19, 683–691 (1984). https://doi.org/10.1007/BF02534528

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  • DOI: https://doi.org/10.1007/BF02534528

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