Abstract
Structural analyses were performed on milk fat samples obtained 3–10 days postpartum from a lactating patient with primary Type 1 hyperlipidemia. The milk triacylglycerols contained 3–7% C10, 14–21% C12, 20–30% C14, 22–26% C16 and 20–30% C18 (largely oleic) acids. Gas liquid chromatographic (GLC) analyses of the X-1,3- and X-1,2-diacylglycerols on polar siloxane columns showed a markedly non-random association of acyl chains. Stereospecific analyses indicated that the short chain length fatty acids were confined essentially to the sn-3-position of the triacylglycerol molecule. Furthermore, these acids were largely absent from the phosphatidylcholines and the endogenous sn-1,2-diacylglycerols of the milk fat. It is concluded that the short chain fatty acids are incorporated into the milk triacylglycerols during the final stage of biosynthesis via the phosphatidic acid pathway, and that the overall fatty acid distribution is consistent with the 1-random 2-random 3-random hypothesis.
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Steiner, G.; Kuksis, A., and Myher, J.J. (1975) Clin. Res. 23, 639A.
Smith, S. and Abraham, S. (1975) Adv. Lipid Res. 13, 195–299.
Pitas, R.D.; Sampugna, J., and Jensen, R.G. (1976) J. Dairy Sci. 50, 1332–1336.
Kuksis, A. (1967) in Lipid Chromatographic Analysis, Vol. 1 (G.V. Marinetti, ed.) Marcel Dekker, New York, NY, pp. 239–337.
Marai, L.; Breckenridge, W.C., and Kuksis, A. (1969) Lipids 4, 562–570.
Folch, J.; Lees, M., and Sloane-Stanley, G.H. (1957) J. Biol. Chem. 226, 497–509.
Myher, J.J. and Kuksis, A. (1975) J. Chromatogr. Sci. 13, 138–145.
Yurkowski, M. and Brockerhoff, H. (1966) Biochim. Biophys. Acta 125, 55–59.
Brockerhoff, H. (1967) J. Lipid Res. 8, 167–169.
Kuksis, A.; Marai, L., and Myher, J.J. (1973) J. Am. Oil Chem. Soc. 50, 193–201.
Breckenridge, W.C. and Kuksis, A. (1967) J. Lipid Res. 8, 473–478.
Steiner, G.; Myher, J.J., and Kuksis, A. (1984) Am. J. Clin. Nutr. In press.
Breckenridge, W.C.; Marai, L., and Kuksis, A. (1969) Can. J. Biochem. 47, 761–769.
Insull, W., Jr.; Hirsch, J.; James, A.T., and Ahrens, E.H., Jr. (1959) J. Clin. Invest. 38, 443–450.
Read, W.W.C.; Lutz, P.G., and Tashjuan, A. (1963) Am. J. Clin. Nutr. 17, 180–183.
Harzer, G.; Haug, M.; Dieterich, I., and Gentner, P.R. (1983) Am. J. Clin. Nutr. 37, 612–621.
Volpe, J.J. and Vagelos, P.R. (1973) Ann. Rev. Biochem. 42, 21–60.
Parodi, P.W. (1982) Lipids 17, 437–442.
Christie, W.W. and Moore, J.H. (1970) Biochim. Biophys. Acta 210, 45–56.
Manganaro, F.; Myher, J.J.; Kuksis, A., and Kritchevsky, D. (1981) Lipids 16, 508–517.
Myher, J.J.; Kuksis, A.; Breckenridge, W.C. and Little, J.A. (1981) Lipids. In Press.
Christie, W.W. (1974) Lipids 9, 876–882.
Morley, N. and Kuksis, A. (1972) J. Biol. Chem. 247, 6389–6393.
Paltauf, F.; Esfandi, F., and Holasek, A. (1974) FEBS Lett. 40, 119–123.
Patton, S. (1973) J. Am. Oil Chem. Soc. 50, 178–185.
Rao, G.A. and Abraham, S. (1983) Lipids 18, 335–338.
Patton, S.; Mumma, R.O., and McCarthy, R.D. (1966) J. Dairy Sci. 49, 937.
Slakey, P.M., and Lands, W.E.M. (1968) Lipids 3, 30–36.
Marshall, M.O. and Knudsen, J. (1977) Biochim. Biophys. Acta 489, 236–241.
Lin, C.Y.; Smith, S., and Abraham, S. (1976) J. Lipid Res. 17, 647–656.
Cooper, S.M. and Grigor, M.R. (1980) Biochem. J. 187, 289–295.
Kinsella, J.E. (1976) Lipids 11, 680–684.
Marshall, M.O. and Knudsen, J. (1979) Eur. J. Biochem. 94, 93–98.
Berger, G.M.B.; Spark, A.; Baillie, P.M.; Huskisson, J.; Stockwell, G., and van de Merve, E. (1983) Pediatr. Res. 17, 835–839.
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Myher, J.J., Kuksis, A. & Steiner, G. Milk fat structure of a patient with type 1 hyperlipidemia. Lipids 19, 673–682 (1984). https://doi.org/10.1007/BF02534527
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DOI: https://doi.org/10.1007/BF02534527