Analyses of lipids and fatty acids in ripe roes of some Northwest European marine fish

Abstract

Lipid class analyses and fatty acid analyses of neutral and polar lipids were carried out on ripe roes of herring, cod, haddock, whiting, saithe, sand eel and capelin. Total lipid was 10–26% of roe dry weight. The species with the highest total lipid, sand eel and capelin, also had the highest percentage of neutral lipid in total lipid, 77% and 49% respectively. In the other species, phospholipids accounted for 62–77% of roe total lipid. Both the neutral lipids, and especially the phospholipids, of all species were very unsaturated because of high concentrations of (n−3) polyunsaturated fatty acids (PUFA), frequently amounting to 50% of the total egg lipid. Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) had similar fatty acid compositions in all species, with an average ratio (n−3)/(n−6) of ca. 20∶1. Phosphatidylinositol (PI) consistently had high concentrations of 18∶0 and 20∶4 (n−6) with an average ratio of (n−3)/(n−6) of 1.8∶1. Requirements for high levels of (n−3) PUFA in the embryonic and early larval development stages of marine fish are suggested as is a special role for the 20∶4(n−6) in PI.

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References

  1. 1.

    Ackman, R.G. (1980) in Advances in Fish Science and Technology (Connell, J.J., ed.), pp. 86–103, Fishing News Book Ltd, Farnham, Surrey, U.K.

    Google Scholar 

  2. 2.

    Sargent, J.R., and Whittle, K.J. (1981) in Analysis of Marine Ecosystems (Longhurst, A.R., ed.) pp. 491–533, Academic Press, London.

    Google Scholar 

  3. 3.

    Watanabe, T. (1982) Comp. Biochem. Physiol. 73B, 3–15.

    CAS  Google Scholar 

  4. 4.

    Bell, M.V., Simpson, C.M.F., and Sargent, J.R. (1983) Lipids, 18, 720–726.

    CAS  Google Scholar 

  5. 5.

    Imada, O., Kageyama, Y., Watanabe, T., Kitajima, C., Fujita, S., and Yone, Y. (1979) Bull. Jap. Soc. Sci. Fish. 45, 955–959.

    CAS  Google Scholar 

  6. 6.

    Watanabe, T., Tamiya, T., Oka, A., Hirata, M., Kitajima, C., and Fujita, S. (1983) Bull. Jap. Soc. Sci. Fish. 49, 471–479.

    CAS  Google Scholar 

  7. 7.

    Lasker, R., and Theilacker, G.H. (1962) J. Lipid Res. 3, 60–64.

    CAS  Google Scholar 

  8. 8.

    Ackman, R.G., and Burgher, R.D. (1964) J. Fish. Res. Bd. Canada 21, 469–476.

    CAS  Google Scholar 

  9. 9.

    Kaitaranta, J.K., and Ackman, R.G. (1981) Comp. Biochem. Physiol. 69B, 725–729.

    CAS  Google Scholar 

  10. 10.

    Wheeler, A. (1969) The Fishes of the British Isles and North-West Europe, MacMillan, London.

    Google Scholar 

  11. 11.

    Folch, J., Lees, M., and Sloane Stanley, G.H. (1957) J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  12. 12.

    Fewster, M.E., Burns, B.J., and Mead, J.F. (1969) J. Chromatogr. 43, 120–126.

    PubMed  CAS  Article  Google Scholar 

  13. 13.

    Parsons, J.G., and Patton, S. (1967) J. Lipid Res. 8, 696–698.

    PubMed  CAS  Google Scholar 

  14. 14.

    Raheja, R.K., Kaur, C., Singh, A., and Bhatia, I.S. (1973) J. Lipid Res. 14, 695–697.

    PubMed  CAS  Google Scholar 

  15. 15.

    Christie, W.W. (1973) in Lipid Analyses, Pergammon Press, Oxford, England.

    Google Scholar 

  16. 16.

    Ackman, R.G., and Eaton, C.A. (1978) Fette Seifen Anstr. Mittel 80, 21–37.

    CAS  Article  Google Scholar 

  17. 17.

    Russell, F.S. (1976) in The Eggs and Planktonic Stages of British Marine Fishes, Academic Press, London, England.

    Google Scholar 

  18. 18.

    Jangaard, P.M. (1974) Bull. Fish. Res. Board Canada No. 186.

  19. 19.

    Boulebache, H. (1981) Amer. Zool. 21, 377–389.

    Google Scholar 

  20. 20.

    Vetter, R.D., Hodson, R.E., and Arnold, C. (1983) Can. J. Fish. Aquatic Sci. 40, 627–634.

    CAS  Google Scholar 

  21. 21.

    Cetta, C.M., and Capuzzo, J.M. (1982) Mar. Biol. 71, 327–337.

    Article  Google Scholar 

  22. 22.

    Cell Calcium: Inositol Phospholipids and Cell Calcium (Michell R.H., ed.) Vol. 3, Churchill Livingstone, Edinburgh and New York.

  23. 23.

    Chacko, G.K., Goldman, D.E., and Pennock, B.E. (1972) Biochim. Biophys. Acta 280, 1–16.

    PubMed  CAS  Google Scholar 

  24. 24.

    Hazel, J.R. (1979) Amer. J. Physiol. 236, R91-R101.

    PubMed  CAS  Google Scholar 

  25. 25.

    Castledine, A.J., and Buckley, J.T. (1982) Comp. Biochem. Physiol. 71B, 119–126.

    CAS  Google Scholar 

  26. 26.

    Cowey, C.B., and Sargent, J.R. (1979) in Fish Physiology, Vol. 8, (Hoar, W.S., Randall, D.J., and Brett, J.R., eds.) pp. 1–69, Academic Press, New York.

    Google Scholar 

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Correspondence to Douglas R. Tocher.

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Tocher, D.R., Sargent, J.R. Analyses of lipids and fatty acids in ripe roes of some Northwest European marine fish. Lipids 19, 492–499 (1984). https://doi.org/10.1007/BF02534481

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Keywords

  • Neutral Lipid
  • Steryl Ester
  • Neutral Lipid Fraction
  • Polar Lipid Fraction
  • Total Polar Lipid