Abstract
A rapid method of isolating a relatively pure fraction of oxygenated fatty acids from plants and natural waters is described. These metabolites were isolated from aqueous extracts using octadecylsilyl silica in a reverse-phase batch extraction method. The extraction method, together with reverse-phase analytical high pressure liquid chromatography (HPLC), was used to establish a routine screening method for the presence of these compounds in a variety of natural sources. A reverse-phase preparative HPLC purification method is also described.
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References
van Aller, R.T., Clark, L.R., Pessoney, G.F., Rogers, V.A. (1983) Lipids 18, 617–622.
Powell, W.S. (1980) Prostaglandins 20, 947–957.
Bennett, H.P.J., Hudson, A.M., McMartin, C., and Purdon, G.E. (1977) Biochem. J. 168, 9.
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Van Rogers, A., Van Aller, R.T., Pessoney, G.F. et al. A rapid procedure for screening and isolating oxygenated free fatty acid metabolites from plants and natural waters. Lipids 19, 304–306 (1984). https://doi.org/10.1007/BF02534461
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DOI: https://doi.org/10.1007/BF02534461