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Phospholipid synthesizing enzymes of dermatophytes III. Glycerol kinase of dermatophytes

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Lipids

Abstract

Glycerol kinase, the key enzyme for glycerol use in phospholipid synthesis, was identified in cytosolic fractions of 2 dermatophytes,Microsporum gypseum andEpidermophyton floccosum. Ammonium sulfate was observed to activate and stabilize this enzyme in both dermatophytes. Two pH optima, 8.0 and 10.5, were observed for both dermatophyte enzymes. Glycerol kinase fromM. gypseum was purified up to 33-fold with a 225% recovery by ammonium sulfate precipitation and gel filtration. The molecular weight of the enzyme was ca. 4.5×105. It had 2 pH optima of 8.0 and 10.5. The enzyme had Km values of 0.35 mM and 2.3 mM for glycerol and ATP. Reactivity of the enzyme for various nucleotides was ATP>TTP>GTP>ITP=CTP=UTP. Kinetic studies showed the enzyme to catalyze the reaction by the ping-pong mechanism. Fructose 1,6-bisphosphate and glucose-6-phosphate inhibited the enzyme competitively, whereas glucose was not inhibitory.

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Authors and Affiliations

  1. Department of Biochemistry, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, India

    C. Kasinathan & G. K. Khuller

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  1. C. Kasinathan
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  2. G. K. Khuller
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Kasinathan, C., Khuller, G.K. Phospholipid synthesizing enzymes of dermatophytes III. Glycerol kinase of dermatophytes. Lipids 19, 289–293 (1984). https://doi.org/10.1007/BF02534457

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  • DOI: https://doi.org/10.1007/BF02534457

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