Abstract
Binding antibodies to surface membranes stimulated incorporation of fatty acids (FA) into phospholipids of L cells. Antibodies stimulated at least a 3.4-fold greater incorporation of arachidonic acid into phosphatidylinositol than into any other class of phospholipid when compared on a molar basis (p<0.003). This enhanced incorpoation was selective, depending on the character of the FA, because antibodies stimulated the incorporation of arachidonic acid at least 2.4-fold more than oleic acid, palmitic acid or stearic acid (p<0.001). Surprisingly, an antibody-stimulated incorporation of palmitic acid into sphingomyelin (SM) was at least 2.2-fold greater than that into any other class of phospholipid (p<0.001) and the antibody-stimulated incorporation of palmitic acid into SM was at least 60-fold greater than that of arachidonic acid, stearic or oleic acid (p<0.001). Nontoxic doses of ethylenediamine tetraacetic acid (EDTA), dexamethasone, 4-bromophenacylbromide and indomethacin inhibited the antibody-stimulated incorporation of arachidonic acid into cellular phospholipids, principally phosphatidylinositol (PI), and similarly inhibited the antibody stimulation of DNA synthesis. We conclude that when antibody binds to surface antigens on L cells, a rapid and selective incorporation of fatty acids into certain cellular phospholipids occurs, possibly mediated by calcium-dependent phospholipases. Degradation products of arachidonic acid, i.e., prostaglandins, may be important in these antibody stimulation events, as well. These early changes in phospholipid metabolism may serve as an important signal or mechanism for the subsequent stimulation of DNA synthesis in L cells.
Similar content being viewed by others
References
Lapetina, E.G., Billah, M.M., and Cuatrecasas, P. (1981) Nature 292, 367–369.
Walsh, C.E., Waite, B.M., Thomas, M.J., and DeChatelet, L.R. (1981) J. Biol. Chem. 256, 7228–7234.
Kennerly, D.A., Sullivan, T.J., and Parker, C.W. (1979) J. Immunol. 122, 152–159.
Schrey, M.P., and Rubin, R.P. (1979) J. Biol. Chem., 254, 11234–11241.
Parker, C.W., Kelly, J.P., Falkenhein, S.F., and Huber, M.G. (1979) J. Exp. Med. 149, 1487–1503.
Habenicht, A.J.R., Glomset, J.A., King, W.C., Nist, C., Mitchell, C.D., and Ross, R. (1981) J. Biol. Chem. 256, 12329–12335.
Hong, S.L., and Deykin, D. (1981) J. Biol. Chem. 256, 5215–5219.
Shearer, W.T., Philpott, G.W., and Parker, C.W. (1973) Science 182, 1357–1359.
Fink, M.P., Parker, C.W., and Shearer, W.T. (1975) Nature 255, 404–405.
Shearer, W.T., Atkinson, J.P., Frank, M.M., and Parker, C.W. (1975) J. Exp. Med. 141, 736–752
Shearer, W.T., Atkinson, J.P., and Parker, C.W. (1976) J. Immunol. 177, 973–980.
Shearer, W.T., and Crouch, J.A. (1977) J. Immunol. 119, 911–917.
Shearer, W.T., and Richards, J.E. (1981) Biochem. Biophys. Res. Commun. 101, 800–806.
Nettleship, A., Earle, W.R., and Shelton, E. (1943) J. Natl. Cancer Inst. 4, 229–234.
McClure, J.A., Bryan, J., Brinkley, B.R., and Shearer, W.T. (1983) Fed. Proc. 42, 917.
Morrisett, J.D., Pownall, H.J., Jackson, R.L., Segura, R., Gotto, Jr., A.M., and Taunton, O.D. (1977) in Polyunsaturated Fatty Acids (Kunau, W.H. and Holman, R.T., eds.) pp. 139–161, American Oil Chemists' Society, Champaign, IL.
Weinstein, D.B., Marsh, J.B., Glick, M.C., and Warren, L.W. (1968) J. Biol. Chem. 244, 4103–4113.
Anderson, R.E., Maude, M.B., and Feldman, G.L. (1969) Biochim. Biophys. Acta 187, 345–353.
Roberts, M.F., Deems, R.A., Mincey, T.C., and Dennis, E.A. (1977) J. Biol. Chem. 252, 2405–2411.
Blackwell, G.J., Flower, R.J., Nijkamp, F.P., and Vane, J.R. (1978) Br. J. Pharmac. 62, 79–89.
Hamberg, M., and Samuelsson, B. (1974) Proc. Natl. Acad. Sci. USA 71, 3400–3404.
Shearer, W.T., Philpott, G.W., and Parker, C.W. (1975) Cell. Immunol. 17, 447–462.
Spector, A.A., Mathur, S.N., Kaduce, T.L., and Hyman, B.T. (1981) Prog. Lipid Res. 19, 155–186.
Holub, B.J., Kuksis, A., and Thompson, W. (1970) J. Lipid Res. 11, 558.
Brockerhoff, H., and Jensen, R.G. (1974) Lipolytic Enzymes, pp. 194–265, Academic Press, New York.
Hofmann, S.L., and Majerus, P.W. (1982) J. Biol. Chem. 257, 6461–6469.
Hofmann, S.L., Prescott, S.M., and Majerus, P.W. (1982) Arch. Biochem. Biophys. 215, 237–244.
Jesse, R.L., and Franson, R.C. (1979) Biochim. Biophys. Acta 575, 467–470.
Rittenhouse-Simmons, S. (1980) J. Biol. Chem. 255, 2259–2262.
Hial, V., DeMello, M.C.F., Horakova, Z., and Beaven, M.A. (1977) J. Pharm. Exp. Ther. 202, 446–454.
Irvine, R.F. (1982) Biochem. J. 204, 3–16.
Hirata, F., Schiffman, E., Venkatasubramanian, K., Solomon, D., and Axelrod, J. (1980) Proc. Natl. Acad. Sci. USA 77, 2533–2536.
Bell, R.L., Kennerly, D.A., Stanford, N., and Majerus, P.W. (1979) Proc. Natl. Acad. Sci. USA 76, 3238–3241.
Flower, R.J. (1981) in The Prostaglandin System (Berti, F. and Velo, G.P., eds.), pp. 27–37, Plenum Press, New York.
Schremmer, J.M., Blank, M.L., and Wykle, R.L. (1979) Prostaglandins 18, 491–504.
Kuksis, A. (1978) Handbook of Lipid Research (Kuksis, A., ed.), Vol. 1, pp. 381–422, Plenum Press, New York.
Rode, H.N., Szamel, M., Schneider, S., and Resch, K. (1982) Biochim. Biophys. Acta 688, 66–74.
Author information
Authors and Affiliations
About this article
Cite this article
Shearer, W.T., Ulrich, R.G. Incorporation of fatty acids into phospholipids in L cells stimulated by antibody. Lipids 19, 239–249 (1984). https://doi.org/10.1007/BF02534451
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534451