Abstract
In a study of lipid-protein interactions in egg yolk, it was found that L-α-dipalmitoyl lecithin gave two distinct noncovalent complexes (A and B) with apovitellenin I, an apoprotein in the major yolk lipoprotein. Interaction took place under widely varied conditions, and yolk lecithin gave similar complexes. Complex A, which was formed within minutes, consisted of round particles of about 9 nm diameter. Complex B, which was formed more slowly, consisted of larger, particles. Possibly resembling curved discs, with diameter of 30–40 nm. The preparation and some properties of these complexes are described. It is suggested that they may be suitable for an extensive study of phospholipid-protein interactions in yolk.
Similar content being viewed by others
References
Cook, W.H., and Martin, W.G. (1969) inStructural and Functional Aspects of Lipoproteins in Living Systems (Tria, E., and Scanu, A.M., eds.) pp. 579–615, Academic Press, New York.
Privett, O.S., Blank, M.L., and Schmit, J.A. (1962)J. Food Sci. 27, 463–468.
Davey, E.M., Zabik, M.E., and Dawson, L.E. (1969)J. Poultry Sci. 48, 251–260.
Osborne, J.C., and Brewer, H.B. (1977)Adv. Protein Chem. 31, 253–337.
Sparrow, J.T., and Gotto, A.M. (1982)Crit. Rev. Biochem. 13, 37–108.
Scanu, A.M., Edestein, C., and Shen, B.W. (1982) inLipid-protein Interactions (Jost, P.C., and Griffith, O.H., eds.) Vol. I, pp. 259–309, John Wiley & Sons, New York.
Dugaiczyk, A., Inglis, A.S., Strike, P.M., Burley, R.W., Beattie, W.G., and Chan, L. (1981)Gene 14, 175–182.
Jackson, R.L., Chan, L., Snow, L.D., and Means, A.R. (1978) inDisturbances in Lipid and Lipoprotein Metabolism (Dietschy, J.M., ed.) pp. 139–154, Williams and Wilkins, Baltimore, MD.
Burley, R.W. (1981)Proc. Aust. Biochem. Soc. 14, 7.
Fretheim, K., and Burley, R.W. (1983)Proc. Aust. Biochem. Soc. 15, 96.
Burley, R.W. (1975)Aust. J. Biol. Sci. 28, 121–132.
Burley, R.W., and Sleigh, R.W. (1983)Biochem. J. 209, 143–150.
Singleton, W.S., Gray, M.S., Grown, M.L., and White, J.L. (1965)J. Amer. Oil Chem. Soc. 42, 53–56.
Fiske, C.H., and Subbarow, Y. (1925)J. Biol. Chem. 66, 375–400.
Smith, M.B., and Rose, S.J. (1975)J. Phys. Ed. 8, 377–382.
Chang, C.T., Wu, C.-S.C., and Yang, J.T. (1978)Anal. Biochem. 91, 13–31.
Segrest, J.P., Jackson, R.L., Morrisett, J.D., and Gotto, A.M. (1974)FEBS Lett. 38, 247–253.
Inglis, A.S., Strike, P.M., and Burley, R.W. (1982)Aust. J. Biol. Sci. 35, 263–269.
Brouillette, C.G., Jones, J.L., Ng, T.C., Kercret, H., Chung, B.H., and Segrest, J.P. (1984)Biochemistry 23, 359–367.
Author information
Authors and Affiliations
About this article
Cite this article
Fretheim, K., Sleigh, R.W. & Burley, R.W. Formation of complexes between lecithin and apovitellenin I, an avian egg-yolk apoprotein. Lipids 21, 127–131 (1986). https://doi.org/10.1007/BF02534433
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534433