Skip to main content
SpringerLink
Log in

Phthalic ester, an artifactual contaminant in diazomethane prepared from N-methyl-N-nitroso-p-toluenesulfonamide for the derivatization of prostaglandins

  • Methods
  • Published:
Lipids

Abstract

During analyses of prostaglandin (PG) methoxime methyl trimethylsilyl (MO-ME-TMS) ether derivatives by gas chromatography, we consistently observed a contaminant peak which we identified as di-2-ethylhexyl phthalate (DEHP). The diazomethane prepared from N-methyl-N-nitroso-p-toluenesulfonamide (MNTSA) using the Diazald kit was the major source of DEHP. Several other materials also were significant sources. The concentrations of DEHP in these materials were quantified by gas chromatography-mass spectrometry.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lands, W.E.M., Ann. Rev. Physiology 41:633 (1979).

    Article  CAS  Google Scholar 

  2. Hwang, D.H., and J.E. Kinsella, Prostaglandins 17:543 (1979).

    Article  PubMed  CAS  Google Scholar 

  3. Fitzpatrick, F.A., Anal. Chem. 50:47 (1978).

    Article  CAS  Google Scholar 

  4. Aldrich Technical Information D2800-0 (1975).

  5. Fales, H.M., T.M. Jaouni and J.F. Babashak, Anal. Chem. 45:2303 (1973).

    Article  Google Scholar 

  6. Fales, H.M., G.W.A. Milne and R.S. Nicholson, Anal. Chem. 43:1785 (1971).

    Article  CAS  Google Scholar 

  7. Autien, J., Environ. Health Pers. (May 3–26, 1973).

  8. Schultz, C.O., and R.J. Rubin, Environ. Health Pers., p. 149 (Jan. 1973).

  9. Asakawa, Y., and G. Genjida, J. Sci. Hiroshima Univ., Ser. A-2 34:103 (1970).

    CAS  Google Scholar 

  10. Friocourt, M.P., F. Berthou, D. Picart, Y. Dreano and H.H. Foloch, J. Chromatogr. 172:261 (1979).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Food Science, Cornell University, 14853, Ithaca, NY

    J. Mai & J. E. Kinsella

  2. Department of Chemistry, Cornell University, 14853, Ithaca, NY

    J. S. Chou

Authors
  1. J. Mai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. E. Kinsella
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. S. Chou
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Mai, J., Kinsella, J.E. & Chou, J.S. Phthalic ester, an artifactual contaminant in diazomethane prepared from N-methyl-N-nitroso-p-toluenesulfonamide for the derivatization of prostaglandins. Lipids 15, 968–971 (1980). https://doi.org/10.1007/BF02534424

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02534424

Keywords

Search

Navigation