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The relative incorporation of arachidonic and eicosapentaenoic acids into human platelet phospholipids

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Lipids

Abstract

The incorporation of arachidonic acid (AA) as compared to eicosapentaenoic acid (EPA) into human platelet phospholipids was tested by incubating washed platelets with a known mixture of [3H]AA and [14C]EPA. Following incubation, the platelet lipids were extracted, the individual phospholipids—phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidylethanolamine (PE)—were separated by thin layer chromatography, and their corresponding [3H]/[14C] ratios were determined. Based on a [3H]/[14C] ratio of unity for the substrate mixture, the PC, PS, PI and PE exhibited ratios of 0.55, 0.93, 1.12 and 0.74, respectively, which were significantly different from 1.00 in all instances except in the case of PS. These results indicate that PC and PE selectively incorporated EPA, while PI showed preference toward AA. These selectivities may account partly for the differing AA/EPA mass ratios that have been observed among the individual phospholipids of human subjects consuming fish oils.

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References

  1. Dyerberg, J., Bang, H.O., Stoffersen, E., Moncada, S., and Vane, J.R. (1978) Lancet 2, 117.

    Article  PubMed  CAS  Google Scholar 

  2. Dyerberg, J., and Bang, H.O. (1979) Lancet 2, 433–435.

    Article  PubMed  CAS  Google Scholar 

  3. Dyerberg, J. (1981) Phil. Trans. R. Soc. Lond. B294, 373–381.

    CAS  Google Scholar 

  4. Sanders, T.A.B., Naismith, D.J., Haines, A.P., and Vickers, M. (1980) Lancet 2, 1189.

    Article  Google Scholar 

  5. Sanders, T.A.B., and Roshanai, F. (1983) Clin. Sci. 64, 91–99.

    PubMed  CAS  Google Scholar 

  6. Sanders, T.A.B., Vickers, M., and Haines, A.P. (1981) Clin. Sci. 61, 317–324.

    PubMed  CAS  Google Scholar 

  7. Seiss, W., Scherer, B., Bohlig, B., Roth, P., Kurzmann, I., and Weber, P.C. (1980) Lancet 1, 441–444.

    Article  Google Scholar 

  8. Ahmed, A.A., and Holub, B.J. (1984) Lipids 19, 617–624.

    PubMed  CAS  Google Scholar 

  9. Goodnight, S.H., Jr., Harris, W.S., and Connor, W.E. (1981) Blood 58, 880–885.

    PubMed  CAS  Google Scholar 

  10. Fischer, S., and Weber, P.C. (1983) Biochem. Biophys. Res. Commun. 116, 1091–1099.

    Article  PubMed  CAS  Google Scholar 

  11. Brox, J.H., Killie, J.E., Gunnes, S., and Nordoy, A. (1981) Thrombosis and Haemostasis 46, 604–611.

    PubMed  CAS  Google Scholar 

  12. Thorngren, M., and Gustafson, A. (1981) Lancet 2, 1190–1193.

    Article  PubMed  CAS  Google Scholar 

  13. Bradlow, B.A., Chetty, N., van der Westhuyzen, J., Mendelsohn, D., and Gibson, J.E. (1983) Thromb. Res. 29, 561–568.

    Article  PubMed  CAS  Google Scholar 

  14. Schick, B.P., Schick, P.K., and Chase, P.R. (1981) Biochim. Biophys. Acta 663, 239–248.

    PubMed  CAS  Google Scholar 

  15. Joist, J.H., Dolezel, G., Lloyd, J.V., and Mustard, J.F. (1976) Blood 48, 199–211.

    PubMed  CAS  Google Scholar 

  16. Bereziat, G., Chambaz, J., Trugnan, G., Pepin, D., and Polonovski, J. (1978) J. Lipid Res. 19, 495–500.

    PubMed  CAS  Google Scholar 

  17. Weaver, B.J., and Holub, B.J. (1985) Nutr. Res. 5, 31–37.

    Article  CAS  Google Scholar 

  18. McKean, M.L., Smith, J.B., and Silver, M.J. (1981) J. Biol. Chem. 256, 1522–1524.

    PubMed  CAS  Google Scholar 

  19. Bligh, E.G., and Dyer, W.J. (1959) Can. J. Biochem. Physiol. 37, 911–918.

    PubMed  CAS  Google Scholar 

  20. Allen, D., and Michell, R.H. (1978) Biochim. Biophys. Acta 508, 277–286.

    Article  Google Scholar 

  21. Steel, R.G.D., and Torrie, J.H. (1980) Principles and Procedures of Statistics, 2nd edn., pp. 86–100, McGraw-Hill Inc., New York.

    Google Scholar 

  22. Rittenhouse-Simmons, S., Russel, F.A., and Deykin, D. (1977) Biochim. Biophys. Acta 488, 370–380.

    PubMed  CAS  Google Scholar 

  23. Bills, T.K., Smith, J.B., and Silver, M.J. (1976) Biochim. Biophys. Acta 424, 303–314.

    PubMed  CAS  Google Scholar 

  24. Weiner, T.W., and Sprecher, H. (1984) Biochim. Biophys. Acta 792, 293–303.

    PubMed  CAS  Google Scholar 

  25. Fischer, S., Schacky, C.V., Seiss, W., Strasser, T., and Weber, P.C. (1984) Biochem. Biophys. Res. Commun. 120, 907–918.

    Article  PubMed  CAS  Google Scholar 

  26. Ahmed, A.A., Mahadevappa, V.G., and Holub, B.J. (1983) Nutr. Res. 3, 673–680.

    Article  CAS  Google Scholar 

  27. Van den Bosch, H., Van Golde, L.M.G., Eibl, H., and van Deenen, L.L.M. (1967) Biochim. Biophys. Acta 144, 613–623.

    PubMed  Google Scholar 

  28. Holub, B.J., and Kuksis, A. (1978) in Advances in Lipid Research (Paoletti, R., and Kritchevsky, D., eds.) Vol. 16, 1–125, Academic Press, New York.

    Google Scholar 

  29. Inoue, M., Murase, S., and Okuyama, H. (1984) Arch. Biochem. Biophys. 231, 29–37.

    Article  PubMed  CAS  Google Scholar 

  30. Iritani, N., Ikeda, Y., and Kajitani, H. (1984) Biochim. Biophys. Acta 793, 416–422.

    PubMed  CAS  Google Scholar 

  31. Wilson, D.B., Prescott, S.M., and Majerus, P.W. (1982) J. Biol. Chem. 257, 3510–3515.

    PubMed  CAS  Google Scholar 

  32. Neufeld, E.J., Wilson, D.B., Sprecher, H., and Majerus, P.W. (1983) J. Clin. Invest. 72, 214–220.

    Article  PubMed  CAS  Google Scholar 

  33. Neufeld, E.J., Sprecher, H., Evans, R.W., and Majerus, P.W. (1984) J. Lipid Res. 25, 288–293.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Nutrition, College of Biological Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    B. J. Weaver & B. J. Holub

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  1. B. J. Weaver
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  2. B. J. Holub
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Weaver, B.J., Holub, B.J. The relative incorporation of arachidonic and eicosapentaenoic acids into human platelet phospholipids. Lipids 20, 773–777 (1985). https://doi.org/10.1007/BF02534401

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  • DOI: https://doi.org/10.1007/BF02534401

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