Abstract
Human plasma of 5 normolipemic individuals was incubated for 24 hr at 37 C in the presence or in the absence of lecithin: cholesterol acyltransferase (LCAT)-inhibitors. Plasma steored at 4 C served as a control. The low density lipoprotein (LDL) fractions of the samples were isolated and investigated with respected to changes in chemical composition and complexing activity with glycosamino glycans (GAG).
Incubation of plasma in the presence of LCAT inhibitors caused a significant increase of LDL triglycerides at the expense of cholesteryl esters. Incubation with active LCAT not only changed the core but also the surface constituents (decrease in phospholipids and in free cholesterol).
The amount of GAG bound per mg of LDL was not uniformly changed in samples incubated after LCAT inhibition. LDL isolated from plasma incubated in the presence of LCAT, on the other hand, showed a significant reduction in GAG binding.
The ratio of free cholesterol: GAG in the complex was most significantly reduced in LCAT-modified LDL. There was in addition a highly significant correlation between the LDL:GAG ratio in the complex and the free cholesterol and phospholipid content of the LDL samples.
It is concluded that alterations in surface lipid constitutents of LDL strongly after their interaction with sulfated polysaccharides, an effect which may be relevant also in vivo for the interaction of LDL with cell surfaces and intercellular matrices.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Castelli, W.P., Doyle, J.T., Gordon, T., Hames, C.G., Hjortland, M.C., Hulley, S.B., Kagan, A., and Zukel, W.Z. (1977) Circulation 55, 767–772.
Bloch, A., Dinsmore, R.E., and Lees, R.S. (1976) Lancet 1, 928–930.
Pilger, E., Pristautz, H., Pfeiffer, K.P., and Kostner, G.M. (1983) Arteriosclerosis 3, 57–63.
Goldstein, J.L., and Brown, M.S. (1977) Ann. Rev. Biochem. 46, 897–930.
Camejo, G. (1982) Advances in Lipid Res. 19, 1–50.
Bernfeld, P., Nisselbaum, J.S., Berkeley, B.J., and Hanson, R.W. (1960) J. Biol. Chem. 235, 2852–2859.
Bihari-Varga, M., Gergely, J., and Gerö, S. (1964) J. Atherosclerosis Res. 4, 106–109.
Bihari-Varga, M. (1965) Acta Chim. 45, 219–230.
Bihari-Varga, M., and Vegh, M. (1967) Biochim. Biophys. Acta 144, 202–210.
Patsch, J., Sailer, S., Kostner, G.M., Sandhofer, F., Holasek, A., and Braunsteiner, H. (1974) J. Lipid Res. 15, 356–366.
Kostner, G.M. (1972) Biochem. J. 130, 913–917.
Bitter, T., and Muir, H. (1962) Analyt. Biochem. 4, 330–334.
Zechner, R., Dieplinger, H., Roscher, A., and Kostner, G.M. (1984) Biochemical J. 224, 569–576.
Glomset, J.A. (1970) J. Clin. Nutrition 23, 1129–1138.
Barter, P.J., Hopkins, G.J., Gorjatschko, L., and Jones, M.E. (1982) Atherosclerosis 44, 27–40.
Zechner, R., Dieplinger, H., Roscher, A., Krempler, F., and Kostner, G.M. (1982) Biochim. Biophys. Acta 712, 433–435.
Nishida, T. (1968) J. Lipid Res. 9, 627–635.
Bihari-Varga, M. (1983) In: Diabetes, Obesity and Hyperlipidemias, 2nd Edition, G. Crepaldi, P.Z. Lefevre, and D.J. Dalton, eds. Acad. Press, New York.
Bihari-Varga, M., Goldstein, M.S., Lagrange, D., and Gruber, E. (1982) Int. J. Biol. Macromol. 4, 438–441.
Teng, G., Thompson, G.R., Sniderman, A.D., Forte, T.M., Krauss, R.M., and Kwiterovich, P.O. (1983) Proc. Natl. Acad. Sci. 80, 6662–6662.
Kostner, G.M., Laggner, P., Prexl, W., and Holasek, A. (1976) Biochem. J. 157, 401–407.
Author information
Authors and Affiliations
About this article
Cite this article
Kostner, G.M., Zechner, R., Bihari-Varga, M. et al. The interaction of human plasma low density lipoproteins with glycosamino-glycans: Influence of the chemical composition. Lipids 20, 24–28 (1985). https://doi.org/10.1007/BF02534358
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534358