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Biosynthesis of 24-methylcholest-5-en-3β-ol and 24-ethylcholest-5-en-3β-ol inZea mays

  • Papers from the H. W. Kircher Memorial Symposium on Chemistry Biosynthesis and Function of Sterols Presented at the 76th AOCS Annual Meeting in Philadelphia, Pennsylvania, May 1985
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Lipids

Abstract

The relative rates of synthesis of 24-methylcholest-5-en-3β-ol and 24-ethylcholest-5-en-3β-ol inZea mays shoots were determined using [2-14C]mevalonic acid and [methyl-14C]methionine as substrates. The 24-ethylsterol had a higher specific activity and it apparently was synthesized at about 3–4 times the rate of the 24-methylsterol.1H NMR spectroscopy showed that the 24-ethylsterol was predominantly the 24α-epimer but the 24-methylsterol was a mixture of the 24α-epimer (30–40%) and the 24β-epimer (60–70%). The results are discussed in relation to the involvement of Δ24(28)-, Δ23, Δ25- and Δ24(25)-sterol intermediates in 24-methyl- and 24-ethylsterol production.

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Author notes

  1. Neil L. A. Misso

    Present address: Department of Plant Physiology, Waite Agricultural Research Institute, University of Adelaide, 5064, Glen Osmond, South Australia

Authors and Affiliations

  1. Department of Biochemistry, University of Liverpool, P. O. Box 147, L69 3BX, Liverpool, UK

    Nigel Rendeli, Neil L. A. Misso & L. John Goad

Authors
  1. Nigel Rendeli
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  2. Neil L. A. Misso
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  3. L. John Goad
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Rendeli, N., Misso, N.L.A. & Goad, L.J. Biosynthesis of 24-methylcholest-5-en-3β-ol and 24-ethylcholest-5-en-3β-ol inZea mays . Lipids 21, 63–68 (1986). https://doi.org/10.1007/BF02534304

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  • DOI: https://doi.org/10.1007/BF02534304

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