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Lipid composition of different areas of murine brain: Effects of lipid extraction procedures

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Lipids

Abstract

The effects of various chemical extraction procedures on the determination of lipid composition of rat and mouse brain have been investigated. Tissue extractions with formic acid/acetone or perchloric acid both resulted in significant losses of total phospholipids and cholesterol. Perchloric acid extraction also degraded, almost quantitatively, ethanolamine plasmalogens to lysophosphatidylethanolamine. Our findings have thus demonstrated that conventional procedures used for extraction of brain tissue for analysis of choline and acetylcholine content cannot also be used for concurrent/simultaneous extraction of phospholipids and cholesterol from the same tissue.

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Authors and Affiliations

  1. Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania

    S. L. Katyal

  2. Department of Psychiatry, Western Psychiatric Institute and Clinic, University of Pittsburgh School of Medicine, 3811 O'Hara Street, 15213, Pittsburgh, PA

    Linda Barilaro & Israel Hanin

Authors
  1. S. L. Katyal
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  2. Linda Barilaro
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  3. Israel Hanin
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Katyal, S.L., Barilaro, L. & Hanin, I. Lipid composition of different areas of murine brain: Effects of lipid extraction procedures. Lipids 20, 201–203 (1985). https://doi.org/10.1007/BF02534255

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  • DOI: https://doi.org/10.1007/BF02534255

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