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Tocopherol-phospholipid liposomes: Maximum content and stability to serum proteins

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Lipids

Abstract

This study addresses two questions: 1) what is the maximum amount of tocopherol that can be contained in egg phosphatidylcholine liposomes, and 2) what is the stability of these vesicles in the presence of serum proteins? These liposomes, made with a French pressure cell, can contain no more than 33 mol % of tocopherol. Tocopherol changes liposomes in a manner similar to cholesterol, making them larger, less permeable to aqueous dyes and highly resistant to protein-induced disruption. The suppression of protein-induced disruption is more pronounced with tocopherol than with cholesterol, even at lower molar ratios. Thus, liposomes containing alpha tocopherol (15 to 30 mol%) may be useful for delivering physiological quantities of this vitamin to cells in culture or to tissues in vivo.

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Authors and Affiliations

  1. Department of Pathology, University of California, San Francisco, CA

    Meredith Halks-Miller

  2. Cardiovascular Research Institute, University of California, San Francisco, CA

    Luke S. S. Guo & Robert L. Hamilton Jr.

  3. Cardiovascular Department of Anatomy, University of California, San Francisco, CA

    Robert L. Hamilton Jr.

Authors
  1. Meredith Halks-Miller
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  2. Luke S. S. Guo
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  3. Robert L. Hamilton Jr.
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Halks-Miller, M., Guo, L.S.S. & Hamilton, R.L. Tocopherol-phospholipid liposomes: Maximum content and stability to serum proteins. Lipids 20, 195–200 (1985). https://doi.org/10.1007/BF02534254

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  • DOI: https://doi.org/10.1007/BF02534254

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