Abstract
Phospholipase A2 (Naja naja naja) catalyzes the hydrolysis of dipalmitoyl phosphatidylcholine in small unilamellar vesicles (SUVs) with a faster initial rate than in large unilamellar vesicles (LUVs) and multilamellar vesicles (MLVs). For the SUVs, the hydrolysis was initially faster for gel phase than liquid crystalline phase phospholipid. For both LUVs and MLVs, hydrolysis was low except in a small temperature range around the thermotropic phase transition of the phospholipid. In this temperature range, the reaction time course of phospholipase action on dipalmitoyl phosphatidylcholine in LUVs and MLVs included a lag period. With SUVs, a lag period also was observed above the phase transition temperature, but it was not observed below it.
Similar content being viewed by others
References
Kensil, C.R., and Dennis, E.A. (1979) J. Biol. Chem. 254, 5843–5848.
Op den Kamp, J.A.F., Kauerz, M.Th., and van Deenen, L.L.M. (1975) Biochim. Biophys. Acta 406, 169–177.
Wilschut, J.C., Regts, J., Westenberg, H., and Scherphof, G. (1978) Biochim. Biophys. Acta 508, 185–196.
Tinker, D.O., Purdon, A.D., Wei, J., and Mason, E. (1978) Can. J. Biochem. 56, 552–558.
Chrzeszczyk, A., Wishnia, A., and Springer, C.S. (1977) Biochim. Biophys. Acta 470, 161–169.
Kremer, J.M.H., and Wiersema, P.H. (1977) Biochim. Biophys. Acta 471, 348–360.
Deems, R.A., and Dennis, E.A. (1981) Methods in Enzymology 71, 703–710.
Darke, P.L., Jarvis, A.A., Deems, R.A., and Dennis, E.A. (1980) Biochim. Biophys. Acta 626, 154–161.
Eaton, B.R., and Dennis, E.A. (1976) Arch. Biochem. Biophys. 176, 604–609.
Huang, C. (1969) Biochemistry 8, 344–352.
Olson, F., Hunt, C.A., Szoka, F.C., Vail, W.J., and Papahadjopolous, D. (1979) Biochim. Biophys. Acta 557, 9–23.
Hinz, H.J., and Sturtevant, J.M. (1972) J. Biol. Chem. 247, 6071–6075.
Blaurock, A.E., and Gamble, R.C. (1979) J. Membrane Biol. 50, 187–204.
Gaber, B.P., and Peticolas, W.L. (1977) Biochim. Biophys. Acta 465, 260–274.
Janiak, M.J., Small, D.M., and Shipley, G.G. (1979) J. Biol. Chem. 254, 6068–6078.
Van Dijck, P.W.M., de Kruiff, B., Aarts, P.A.M.M., Verkleij, A.J., and de Gier, J. (1978) Biochim. Biophys. Acta 506, 183–191.
Lichtenberg, D., Menashe, M., Donaldson, S., and Biltonen, R.L. (1984) Lipids 19, 395–400.
Menashe, M., Lichtenberg, D., Gutierrez Merino, C., and Biltonen, R.L. (1981) J. Biol. Chem. 256, 4541–4543.
Kensil, C.R., and Dennis, E.A. (1981) Biochemistry 21, 6079–6085.
Dennis, E.A. (1983) The Enzymes 16, 307–353.
Verger, R., Mieras, M.C.E., and de Haas, G.H. (1973) J. Biol. Chem. 248, 4023–4034.
Kantor, H.L., and Prestegard, J.H. (1978) Biochemistry 17, 3592–3597.
Author information
Authors and Affiliations
About this article
Cite this article
Kensil, C.R., Dennis, E.A. Action of cobra venom phospholipase A2 on large unilamellar vesicles: Comparison with small unilamellar vesicles and multibilayers. Lipids 20, 80–83 (1985). https://doi.org/10.1007/BF02534212
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534212