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Phosphatidylinositol metabolism during fertilization in the sea urchin egg

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Lipids

Abstract

Fertilization of the sea urchin egg results in a transient decline in the amount of phosphatidylinositol (PI) to a level equal to about 50% of that present in the unfertilized egg. This response begins as early as 15 seconds after insemination. The level of PI reaches a minimum at 30 seconds post-insemination, and returns to the original value between 2 and 5 min later. Pulse labelling studies with32PO4 and [3H]-inositol showed that the incorporation of these two isotopes into 1-(3-sn-phosphatidyl)-L-myoinositol 4,5-biphosphate [PtdIns(4,5)P2] increased as much as 50% within one minute after insemination. This suggests that at least part of the reduction in PI levels represents the phosphorylation of PI to form PtdIns(4,5)P2. We also found that the production of [3H]-labelled 1D-myoinositol 1,4,5 triphosphate [Ins(1,4,5)P3] present in the trichloroacetic acid (TCA) soluble fraction of eggs increased over five-fold during the first 10 min post insemination. The temporal correlation between the early burst of PtdIns(4,5)P2 and Ins(1,4,5)P3 formation and the transient increase in intracellular free calcium known to occur in the fertilized egg suggest that the production of PtdIns(4,5)P2 and ultimately Ins(1,4,5)P3 may be associated with calcium mobilization within the egg.

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References

  1. Chambers, E.L., and White, W.E. (1954) Biol. Bull. 106, 297–307.

    CAS  Google Scholar 

  2. Epel, D. (1972) Exp. Cell Res. 72, 74–89.

    Article  PubMed  CAS  Google Scholar 

  3. Epel, D., Patton, C., Wallace, R.W. and Cheung, W.Y. (1981) Cell 23, 543–549.

    Article  PubMed  CAS  Google Scholar 

  4. Keller, C., Gundersen, G., and Shapiro, B.M. (1980) Devel. Biol. 74, 86–100.

    Article  CAS  Google Scholar 

  5. Ribot, H., Eisenman, E., and Kinsey, W.H. (1984) J. Biol. Chem. 259, 5333–5338.

    PubMed  CAS  Google Scholar 

  6. Shen, S.S., and Steinhardt, R.A. (1978) Nature (Lond.) 272, 253–254.

    Article  CAS  Google Scholar 

  7. Epel, D., Steinhardt, R.A., Humphreys, T., and Mazia, D. (1974) Dev. Biol. 40, 245–255.

    Article  PubMed  CAS  Google Scholar 

  8. Mazia, D., and Ruby, A. (1974) Exp. Cell Res. 85, 167–172.

    Article  PubMed  CAS  Google Scholar 

  9. Chambers, E.L., and deArmendi, J. (1979) Exp. Cell Res. 122, 203–218.

    Article  PubMed  CAS  Google Scholar 

  10. Steinhardt, R., Zucker, R., and Schatten, G. (1977) Dev. Biol. 58, 185–196.

    Article  PubMed  CAS  Google Scholar 

  11. Epel, D. (1975) Amer. Zool. 15, 507–522.

    Google Scholar 

  12. Shapiro, B.M. (1978) Horizons in Biochemistry and Biophysics, Vol. 4, pp. 201–242, Addison Wesley Co., Reading, MA.

    Google Scholar 

  13. Foerder, C.A., Klebanoff, S.J., and Shapiro, B.M. (1978) Proc. Nat. Acad. Sci. 75, 3183–3187.

    Article  PubMed  CAS  Google Scholar 

  14. Steinhardt, R., and Epel, D. (1974) Proc. Nat. Acad. Sci. 71, 1915–1919.

    Article  PubMed  CAS  Google Scholar 

  15. Chambers, E.L., Pressman, B.C., and Rose, B. (1974) Biochem. Biophys. Res. Comm. 60, 126–132.

    Article  PubMed  CAS  Google Scholar 

  16. Chambers, E.L., and Hinkley, R.E. (1979) Exp. Cell Res. 124, 441–446.

    Article  PubMed  CAS  Google Scholar 

  17. Brandiff, B., Hinegardner, R.T., and Steinhardt, R. (1975) J. Exp. Zool. 192, 13–24.

    Article  Google Scholar 

  18. Fain, J.N., and Berridge, M.J. (1979) Biochem. J. 178, 45–58.

    PubMed  CAS  Google Scholar 

  19. Godfrey, P.P., and Putney, J.W. Jr. (1984) Biochem. J. 218, 187–195.

    PubMed  CAS  Google Scholar 

  20. Streb, H., Irvine, R.F., Berridge, M.J., and Schulz, I. (1983) Nature (Lond.) 306, 67–69.

    Article  CAS  Google Scholar 

  21. Creba, J.A., Downes, C.P., Hawkins, P.T., Brewster, G., Michell, R.H., and Kirk, C.J. (1983) Biochem. J. 212, 733–747.

    PubMed  CAS  Google Scholar 

  22. Joseph, S.K., Thomas, A.P., Williams, R.J., Irvine, R.F., and Williamson, J.R. (1984) J. Biol. Chem. 259, 3077–3081.

    PubMed  CAS  Google Scholar 

  23. Prescott, S.M., and Majerus, P.W. (1981) J. Biol. Chem. 256, 579–582.

    PubMed  CAS  Google Scholar 

  24. Orchard, J.L., Davis, J.S., Larson, R.E., and Farese, R.E. (1984) Biochem. J. 217, 281–287.

    PubMed  CAS  Google Scholar 

  25. Berridge, M.J., Dawson, R.M.C., Downes, C.P., Heslop, J.P., and Irvine, R.F. (1983) Biochem. J. 212, 473–482.

    PubMed  CAS  Google Scholar 

  26. Bartlett, G. (1959) J. Biol. Chem. 234, 446–460.

    Google Scholar 

  27. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  28. Schmell, E., and Lennarz, W.L. (1974) Biochemistry. Vol. 13, pp. 4114–4121.

    Article  PubMed  CAS  Google Scholar 

  29. Eisen, A., Kiehart, D.P., Wieland, S.J., and Reynolds, G.T. (1984) J. Cell Biol. 99, 1647–1654.

    Article  PubMed  CAS  Google Scholar 

  30. Schuel, H. (1984) Biol. Bull. 167, 271–309.

    CAS  Google Scholar 

  31. Berridge, M.J. (1981) Mol. Cell. Endocrinol. 24, 115–140.

    Article  PubMed  CAS  Google Scholar 

  32. Berridge, M.J. (1983) Biochem. J. 212, 849–858.

    PubMed  CAS  Google Scholar 

  33. Hesketh, R. (1983) Nature (Lond.) 306, 16–17.

    Article  CAS  Google Scholar 

  34. Bell, R.L., and Majerus, P.W. (1980) J. Biol. Chem. 255, 1790–1792.

    PubMed  CAS  Google Scholar 

  35. Hong, S.L., and Deykin, D. (1981) J. Biol. Chem. 256, 5215–5219.

    PubMed  CAS  Google Scholar 

  36. Benabe, J.E., Spry, L.A., and Morrison, A.R. (1982) J. Biol. Chem. 257, 7430–7434.

    PubMed  CAS  Google Scholar 

  37. Ferguson, J.E., and Shen, S.S. (1984) Gam. Res. 9, 329–338.

    Article  CAS  Google Scholar 

  38. Perry, G., and Epel, D. (1984) Devel. Biol. 107, 58–65.

    Article  Google Scholar 

  39. Schuel, H., Traeger, E., Schuel, R., and Boldt, J. (1984) Gamete Res. 10, 9–19.

    Article  CAS  Google Scholar 

  40. Moss, R., Schuel, R., and Schuel, H. (1983) Biol. Bull. 165, 516–521.

    Google Scholar 

  41. Turner, P.R., Sheetz, M.P., and Jaffe, L. (1984) Nature 310, 414–415.

    Article  PubMed  CAS  Google Scholar 

  42. Azarnia, R., and Chambers, E.L. (1976) J. Exp. Zool. 198, 65–78.

    Article  PubMed  CAS  Google Scholar 

  43. Whitaker, M., and Irvine, R.F. (1984) Nature 312, 636–639.

    Article  CAS  Google Scholar 

  44. Kishimoto, A., Takai, Y., Mori, T., Kikkawa, U., and Nishizurka, Y. (1979) J. Biol. Chem. 255, 2273–2276.

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, University of Miami School of Medicine, P.O. Box 016960, 33101, Miami, FL

    L. Christopher Kamel, Judy Bailey, Laura Schoenbaum & William Kinsey

Authors
  1. L. Christopher Kamel
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  2. Judy Bailey
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  3. Laura Schoenbaum
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  4. William Kinsey
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Kamel, L.C., Bailey, J., Schoenbaum, L. et al. Phosphatidylinositol metabolism during fertilization in the sea urchin egg. Lipids 20, 350–356 (1985). https://doi.org/10.1007/BF02534201

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  • DOI: https://doi.org/10.1007/BF02534201

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