Abstract
Macrophomina phaseolina was grown on a defined medium at three different carbon/nitrogen ratios. The lipids of the mycelia and the sclerotia were extracted; fractionated into polarity groups; and separated by thin layer, column, and gas liquid chromatographies. Sclerotia contained higher levels of neutral lipids and lower amounts of polar lipids than mycelia. The neutral lipid content of sclerotia increased, up to 77% of total lipids, and phospholipids decreased as carbon/nitrogen ratio increased from 10 to 320. The glycolipid content was not altered significantly by changes in carbon/nitrogen ratios. Although cardiolipin could not be detected in sclerotial polar lipids, both sclerotia and mycelia contained similar phospholipid profiles with major quantitative differences. Phosphatidic acid and phosphatidyl glycerol were major components of sclerotia, whereas phosphatidyl ethanolamine and phosphatidyl inositol were the major phosphatides of mycelia. Phosphatidyl choline was present in both mycelia and sclerotia. The fatty acid distribution did not show any particular pattern of saturation or unsaturation due to differences in carbon/nitrogen ratio. However, mycelial lipids tended to contain C24∶1, C18∶3, and C22∶1 as major fatty acids, whereas the major fatty acids in sclerotial lipids were C18∶2, C18∶1, C22∶1, C20∶0, and C16∶1. Saturated fatty acids were present in lesser concentrations.
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References
Wyllie, T.D., and M.F. Brown, Jr., Phytopathology 60:524 (1970).
Bharucha, K.E., and F.D. Gunstone, J. Chem. Soc. 123:610 (1957).
Morris, L.J., S.W. Hall, and A.T. James, Biochem. J. 100:29C (1966).
Thiele, O.W., Biochim. Biophys. Acta 84:483 (1964).
Morris, L.J., Lipids 3:260 (1968).
Howell, D. McB., and C.L. Fergus, Can. J. Microbiol. 10:616 (1964).
Sumner, J.L., and N. Colotelo, Ibid. 16:1171 (1970).
Kates, M., in “Lipid Chromatographic Analysis,” Edited by G.V. Marinetti, Marcel Dekker, New York, N.Y., 1967, pp. 1–39.
Wassef, M.K., and J.W. Hendrix, Can. J. Botany 52:1123 (1974).
Rouser, G., G. Kritchevsky, and A. Yamamoto, in “Lipid Chromatographic Analysis,” Edited by G.V. Marinetti, Marcel Dekker, New York, N.Y., 1967, pp. 99–162.
Marinetti, G.V., J. Lipid Res. 3:1 (1962).
Marinetti, G.V., in “Biochemical Separations,” Edited by A.T. James and L.J. Morris, Van Nostrand, Prinston, N.J., 1964, p. 339.
Kates, M., in “Laboratory Techniques in Biochemistry and Molecular Biology,” Edited by T.S. Work and E. Work, American Elsevier, New York, N.Y., 1972, p. 269.
Dittmer, J.C., and M.A. Wells, in “Methods of Enzymology,” Vol. 14, Edited by S.P. Colowick and N.O. Kaplan, Academic Press, New York, N.Y., 1969, pp. 482–530.
Rouser, G., A.N. Siakotos, and S. Fleischer, Lipids 1:85 (1966).
Shaw, R., Comp. Biochem. Physiol. 18:325 (1966).
Shaw, R., Biochim. Biophys. Acta 98:230 (1965).
Hulanicka, D., J.A. Erwin, and K. Bloch, J. Biol. Chem. 239:2778 (1964).
Erwin, J.A., and K. Bloch, Ibid. 238:1618 (1963).
McElroy, F.A., and H.B. Stewart, Can. J. Biochem. 45:171 (1967).
Bentley, R., W.V. Lavate, and C.C. Sweeley, Comp. Biochem. Physiol. 11:263 (1964).
Leegwater, D.G., C.G. Youngs, J.F.T. Spencer, and B.M. Craig, Can. J. Biochem. Physiol. 40:847 (1962).
Talbot, G., and L.C. Vining, Can. J. Botany 41:639 (1963).
Tyrrell, D., Can. J. Microbiol. 15:818 (1969).
Korn, E.D., C.L. Greenblatt, and A.M. Lees, J. Lipid Res. 6:34 (1965).
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Paper no 75-11-25 of the Kentucky Agricultural Experiment Station.
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Wassef, M.K., Ammon, V. & Wyllie, T.D. Polar lipids ofMacrophomina phaseolina . Lipids 10, 185–190 (1975). https://doi.org/10.1007/BF02534157
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DOI: https://doi.org/10.1007/BF02534157